Rodyti trumpą aprašą

dc.contributor.authorVoronovič, Evelina
dc.contributor.authorSkripka, Artiom
dc.contributor.authorJarockytė, Greta
dc.contributor.authorGer, Marija
dc.contributor.authorKučiauskas, Dalius
dc.contributor.authorKaupinis, Algirdas
dc.contributor.authorValius, Mindaugas
dc.contributor.authorRotomskis, Ričardas
dc.contributor.authorVetrone, Fiorenzo
dc.contributor.authorKarabanovas, Vitalijus
dc.date.accessioned2023-09-18T16:08:31Z
dc.date.available2023-09-18T16:08:31Z
dc.date.issued2021
dc.identifier.issn1944-8244
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/111715
dc.description.abstractFluorophores with multifunctional properties known as rare-earth-doped nanoparticles (RENPs) are promising candidates for bioimaging, therapy, and drug delivery. When applied in vivo, these nanoparticles (NPs) have to retain long blood-circulation time, bypass elimination by phagocytic cells, and successfully arrive at the target area. Usually, NPs in a biological medium are exposed to proteins, which form the so-called “protein corona” (PC) around the NPs and influence their targeted delivery and accumulation in cells and tissues. Different surface coatings change the PC size and composition, subsequently deciding the fate of the NPs. Thus, detailed studies on the PC are of utmost importance to determine the most suitable NP surface modification for biomedical use. When it comes to RENPs, these studies are particularly scarce. Here, we investigate the PC composition and its impact on the cellular uptake of citrate-, SiO2-, and phospholipid micelle-coated RENPs (LiYF4:Yb3+,Tm3+). We observed that the PC of citrate- and phospholipid-coated RENPs is relatively stable and similar in the adsorbed protein composition, while the PC of SiO2-coated RENPs is larger and highly dynamic. Moreover, biocompatibility, accumulation, and cytotoxicity of various RENPs in cancer cells have been evaluated. On the basis of the cellular imaging, supported by the inhibition studies, it was revealed that RENPs are internalized by endocytosis and that specific endocytic routes are PC composition dependent. Overall, these results are essential to fill the gaps in the fundamental understanding of the nano-biointeractions of RENPs, pertinent for their envisioned application in biomedicine.eng
dc.formatPDF
dc.format.extentp. 39076-39087
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyScopus
dc.titleUptake of upconverting nanoparticles by breast cancer cells: surface coating versus the protein corona
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references70
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionNacionalinis vėžio institutas Vilniaus universitetas Vilniaus Gedimino technikos universitetas
dc.contributor.institutionUniversité du Québec
dc.contributor.institutionNacionalinis vėžio institutas
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionNacionalinis vėžio institutas Vilniaus universitetas
dc.contributor.institutionNacionalinis vėžio institutas Vilniaus Gedimino technikos universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldN 011 - Biofizika / Biophysics
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.researchfieldT 008 - Medžiagų inžinerija / Material engineering
dc.subject.studydirectionD05 - Biofizika / Biophysics
dc.subject.studydirectionF03 - Medžiagų technologijos / Materials technology
dc.subject.studydirectionF05 - Biotechnologijos / Biotechnology
dc.subject.vgtuprioritizedfieldsFM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enlithium yttrium fluoride (LiYF4)
dc.subject.enrare-earth-doped nanoparticles
dc.subject.enprotein corona
dc.subject.encellular uptake
dc.subject.enendocytosis
dc.subject.enupconversion
dcterms.sourcetitleACS applied materials and interfaces
dc.description.issueiss. 33
dc.description.volumevol. 13
dc.publisher.nameACS publications
dc.publisher.cityWashington
dc.identifier.doi000691785200013
dc.identifier.doi10.1021/acsami.1c10618
dc.identifier.elaba102031187


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