dc.contributor.author | Kiełbik, Aleksander | |
dc.contributor.author | Szlasa, Wojciech | |
dc.contributor.author | Novickij, Vitalij | |
dc.contributor.author | Szewczyk, Anna | |
dc.contributor.author | Maciejewska, Magdalena | |
dc.contributor.author | Saczko, Jolanta | |
dc.contributor.author | Kulbacka, Julita | |
dc.date.accessioned | 2023-09-18T16:08:44Z | |
dc.date.available | 2023-09-18T16:08:44Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | (SCOPUS_ID)85111960938 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/111760 | |
dc.description.abstract | Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric fields (nsPEFs) bursts proved to enhance the effectiveness of the therapy. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Herein we present the in vitro study for developing applications based on this new technology. In this study, we used flow cytometric analysis, cell viability assay, caspase activity analysis, wound healing assay, confocal microscopy study, and immunofluorescence to investigate the biological effect of high-frequency nsPEFs on PCa cells. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The cytotoxicity is significantly enhanced in MHz compression of pulses and with the presence of extracellular Ca2+. High-frequency nsPEFs trigger changes in PCa cells’ cytoskeleton and their mobility. The presented data show a therapeutic potential of high-frequency nsPEFs in a PCa setting. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-10 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | DOAJ | |
dc.relation.isreferencedby | PubMed | |
dc.relation.isreferencedby | PubMed Central | |
dc.relation.isreferencedby | Dimensions | |
dc.source.uri | https://doi.org/10.1038/s41598-021-95180-7 | |
dc.title | Effects of high-frequency nanosecond pulses on prostate cancer cells | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | |
dcterms.license | Creative Commons – Attribution – 4.0 International | |
dcterms.references | 46 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Medical University Hospital Wroclaw Medical University | |
dc.contributor.institution | Wroclaw Medical University | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Wroclaw Medical University University of Wroclaw | |
dc.contributor.institution | Polish Academy of Sciences | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | T 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.vgtuprioritizedfields | MC0404 - Bionika ir biomedicinos inžinerinės sistemos / Bionics and Biomedical Engineering Systems | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dcterms.sourcetitle | Scientific reports | |
dc.description.volume | vol. 11 | |
dc.publisher.name | Nature Publishing Group | |
dc.publisher.city | London | |
dc.identifier.doi | 2-s2.0-85111960938 | |
dc.identifier.doi | 85111960938 | |
dc.identifier.doi | 1 | |
dc.identifier.doi | 000684831900008 | |
dc.identifier.doi | 10.1038/s41598-021-95180-7 | |
dc.identifier.elaba | 102444129 | |