dc.contributor.author | Jurgutis, Džiugas | |
dc.contributor.author | Jarockytė, Greta | |
dc.contributor.author | Poderys, Vilius | |
dc.contributor.author | Dodonova-Vaitkūnienė, Jelena | |
dc.contributor.author | Tumkevičius, Sigitas | |
dc.contributor.author | Vyšniauskas, Aurimas | |
dc.contributor.author | Rotomskis, Ričardas | |
dc.contributor.author | Karabanovas, Vitalijus | |
dc.date.accessioned | 2023-09-18T16:17:20Z | |
dc.date.available | 2023-09-18T16:17:20Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.other | (crossref_id)137350149 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/112809 | |
dc.description.abstract | BODIPY-based molecular rotors are highly attractive imaging tools for imaging intracellular microviscosity in living cells. In our study, we investigated the ability to detect the microviscosity of biological objects by using BDP-NO2 and BDP-H molecular rotors. We describe in detail the optical properties of BDP-NO2 and BDP-H molecular rotors in aqueous media with and without proteins, together with their accumulation dynamics and localization in live and fixed human breast cancer cells. Furthermore, we investigate the applicability of these molecules to monitor microviscosity in the organelles of human breast cancer cells by fluorescence lifetime imaging microscopy (FLIM). We demonstrate that the BDP-NO2 molecular rotor aggregates in aqueous media and is incompatible with live cell imaging. The opposite effect is observed with BDP-H which preserves its stability in aqueous media, diffuses through the plasma membrane and accumulates in lipid droplets (LDs) and the cytosol of both live and fixed MCF-7 and MDA-MB-231 cancer cells. Finally, by utilizing BDP-H we demonstrate that LD microviscosity is significantly elevated in more malignant MDA-MB-231 human breast cancer cells, as compared to MCF-7 breast cancer cells. Our findings demonstrate that BDP-H is a water-compatible probe that can be successfully applied to measure microviscosity in the LDs of living cells. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-17 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | DOAJ | |
dc.relation.isreferencedby | J-Gate | |
dc.relation.isreferencedby | CABI (abstracts) | |
dc.relation.isreferencedby | PubMed | |
dc.rights | Laisvai prieinamas internete | |
dc.source.uri | https://www.mdpi.com/1422-0067/23/10/5687/htm | |
dc.source.uri | https://talpykla.elaba.lt/elaba-fedora/objects/elaba:130619338/datastreams/MAIN/content | |
dc.title | Exploring BODIPY-Based sensor for Imaging of intracellular microviscosity in human breast cancer cells | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) | |
dcterms.license | Creative Commons – Attribution – 4.0 International | |
dcterms.references | 66 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Nacionalinis vėžio institutas Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras | |
dc.contributor.institution | Nacionalinis vėžio institutas | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras | |
dc.contributor.institution | Nacionalinis vėžio institutas Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
dc.subject.researchfield | N 002 - Fizika / Physics | |
dc.subject.researchfield | N 003 - Chemija / Chemistry | |
dc.subject.researchfield | T 008 - Medžiagų inžinerija / Material engineering | |
dc.subject.vgtuprioritizedfields | FM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | microviscosity | |
dc.subject.en | molecular rotors | |
dc.subject.en | fluorescence spectroscopy | |
dc.subject.en | live cancer cells | |
dc.subject.en | lipid droplets | |
dc.subject.en | fluorescence lifetime imaging | |
dcterms.sourcetitle | International journal of molecular sciences | |
dc.description.issue | iss. 10 | |
dc.description.volume | vol. 23 | |
dc.publisher.name | MDPI | |
dc.publisher.city | Basel | |
dc.identifier.doi | 137350149 | |
dc.identifier.doi | 000803406700001 | |
dc.identifier.doi | 10.3390/ijms23105687 | |
dc.identifier.elaba | 130619338 | |