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dc.contributor.authorVaitiekūnaitė, Dorotėja
dc.contributor.authorBružaitė, Ingrida
dc.contributor.authorSnitka, Valentinas
dc.date.accessioned2023-09-18T16:17:51Z
dc.date.available2023-09-18T16:17:51Z
dc.date.issued2022
dc.identifier.issn1386-1425
dc.identifier.other(PMID)35334429
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/112923
dc.description.abstractBlueberries (Vaccinium sp.) are consumed all around the globe, however, their endophytic community has not been thoroughly researched, specifically their fruit endophytes. We aimed to isolate and analyze easily cultivable blueberry fruit endophytes to help in future research, concerning probiotic microorganisms. Twelve strains were isolated in this pilot study, genetically homologous with Staphylococcus hominis, Staphylococcus cohnii, Salmonella enterica, Leuconostoc mesenteroides, and [Candida] santamariae. To determine the molecular composition of these isolates we used label-free surface-enhanced Raman spectroscopy (SERS). To our knowledge, this is the first time that SERS spectra for L. mesenteroides and C. santamariae are presented, as well as the first report of Candida yeast, isolated specifically from blueberry fruits. Our findings suggest that the differences in tested yeast and bacteria SERS spectra and subsequent differentiation are facilitated by minor shifts in spectral peak positions as well as their intensities. Moreover, we used principal component and discriminant function analyses to differentiate chemotypes within our isolate group, proving the sensitivity of the technique and its usefulness to recognize different strains in plant-associated microbe samples, which will aid to streamline future studies in biofertilizers and biocontrol agents.eng
dc.formatPDF
dc.format.extentp. 1-11
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyMEDLINE
dc.source.urihttps://www.sciencedirect.com/science/article/pii/S1386142522003079?via%3Dihub
dc.titleEndophytes from blueberry (Vaccinium sp.) fruit: characterization of yeast and bacteria via label-free surface-enhanced Raman spectroscopy (SERS)
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references103
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionLietuvos agrarinių ir miškų mokslų centras
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionKauno technologijos universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldN 002 - Fizika / Physics
dc.subject.researchfieldA 004 - Miškotyra / Forestry
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.enbacteria
dc.subject.enendophytes
dc.subject.enfruit
dc.subject.ensurface-enhanced Raman spectroscopy
dc.subject.envaccinium
dc.subject.enyeast
dcterms.sourcetitleSpectrochimica acta Part A: Molecular and biomolecular spectroscopy
dc.description.volumevol. 275
dc.publisher.nameElsevier
dc.publisher.cityOxford
dc.identifier.doi35334429
dc.identifier.doi2-s2.0-85126663766
dc.identifier.doi000820827500009
dc.identifier.doi10.1016/j.saa.2022.121158
dc.identifier.elaba124956256


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