dc.contributor.author | Ravoitytė, Bazilė | |
dc.contributor.author | Lukša, Juliana | |
dc.contributor.author | Wellinger, Ralf Erik | |
dc.contributor.author | Serva, Saulius | |
dc.contributor.author | Servienė, Elena | |
dc.date.accessioned | 2023-09-18T16:18:15Z | |
dc.date.available | 2023-09-18T16:18:15Z | |
dc.date.issued | 2022 | |
dc.identifier.other | (crossref_id)136262777 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/113001 | |
dc.description.abstract | Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host’s gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-24 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | Scopus | |
dc.rights | Laisvai prieinamas internete | |
dc.source.uri | https://talpykla.elaba.lt/elaba-fedora/objects/elaba:127990210/datastreams/MAIN/content | |
dc.title | Adaptive response of Saccharomyces hosts to Totiviridae L-A dsRNA viruses Is achieved through intrinsically balanced action of targeted transcription factors | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 120 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Gamtos tyrimų centras | |
dc.contributor.institution | Universidad de Sevilla-CSIC | |
dc.contributor.institution | Vilniaus universitetas Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Gamtos tyrimų centras Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.en | Totiviridae | |
dc.subject.en | dsRNA | |
dc.subject.en | transcription | |
dc.subject.en | yeast | |
dc.subject.en | Saccharomyces cerevisiae | |
dc.subject.en | Saccharomyces paradoxus | |
dcterms.sourcetitle | Journal of fungi | |
dc.description.issue | iss. 4 | |
dc.description.volume | vol. 8 | |
dc.publisher.name | MDPI AG | |
dc.publisher.city | Basel | |
dc.identifier.doi | 136262777 | |
dc.identifier.doi | 000786300000001 | |
dc.identifier.doi | 10.3390/jof8040381 | |
dc.identifier.elaba | 127990210 | |