dc.contributor.author | Šilkūnas, Mantas | |
dc.contributor.author | Gudvangen, Emily | |
dc.contributor.author | Novickij, Vitalij | |
dc.contributor.author | Pakhomov, Andrei G. | |
dc.date.accessioned | 2023-09-18T16:22:12Z | |
dc.date.available | 2023-09-18T16:22:12Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0005-2736 | |
dc.identifier.other | (SCIDIR_EID)1-s2.0-S0005273622001729 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/113506 | |
dc.description.abstract | Neuromodulation applications of nanosecond electric pulses (nsEP) are hindered by their low potency to elicit action potentials in neurons. Excitation by a single nsEP requires a strong electric field which injures neurons by electroporation. We bypassed the high electric field requirement by replacing single nsEP stimuli with high-frequency brief nsEP bursts. In hippocampal neurons, excitation thresholds progressively decreased at nsEP frequencies above 20–200 kHz, with up to 20–30-fold reduction at sub-MHz and MHz rates. For a fixed burst duration, thresholds were determined by the duty cycle, irrespective of the specific nsEP duration, rate, or number of pulses per burst. For 100-μs bursts of 100-, 400-, or 800-ns pulses, the threshold decreased as a power function when the duty cycle exceeded 3–5 %. nsEP bursts were compared with single “long” pulses whose duration and amplitude matched the duration and the time-average amplitude of the burst. Such pulses deliver the same electric charge as bursts, within the same time interval. High-frequency nsEP bursts excited neurons at the time-average electric field 2–3 times below the threshold for a single long pulse. For example, the excitation threshold of 139 ± 14 V/cm for a single 100-μs pulse decreased to 57 ± 8 V/cm for a 100-μs burst of 100-ns, 0.25-MHz pulses (p < 0.001). Applying nsEP in bursts reduced or prevented the loss of excitability in multiple stimulation attempts. Stimulation by high-frequency nsEP bursts is a powerful novel approach to excite neurons at paradoxically low electric charge while also avoiding the electroporative membrane damage. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-8 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | ScienceDirect | |
dc.title | Sub-MHz bursts of nanosecond pulses excite neurons at paradoxically low electric field thresholds without membrane damage | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 41 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Old Dominion University Lietuvos sveikatos mokslų universitetas | |
dc.contributor.institution | Old Dominion University | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | T 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.vgtuprioritizedfields | MC0505 - Inovatyvios elektroninės sistemos / Innovative Electronic Systems | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | nanosecond pulses | |
dc.subject.en | electroporation | |
dc.subject.en | electrostimulation | |
dc.subject.en | neuromodulation | |
dc.subject.en | excitation | |
dc.subject.en | membrane permeability | |
dcterms.sourcetitle | Biochimica et Biophysica Acta (BBA) - Biomembranes | |
dc.description.issue | iss. 11 | |
dc.description.volume | vol. 1864 | |
dc.publisher.name | Elsevier | |
dc.publisher.city | Amsterdam | |
dc.identifier.doi | 1-s2.0-S0005273622001729 | |
dc.identifier.doi | S0005-2736(22)00172-9 | |
dc.identifier.doi | 85135905365 | |
dc.identifier.doi | 2-s2.0-85135905365 | |
dc.identifier.doi | 0 | |
dc.identifier.doi | S0005273622001729 | |
dc.identifier.doi | 139658282 | |
dc.identifier.doi | 000861198100004 | |
dc.identifier.doi | 10.1016/j.bbamem.2022.184034 | |
dc.identifier.elaba | 139064465 | |