dc.contributor.author | Grybchuk, Danyil | |
dc.contributor.author | Akopyants, Natalia S | |
dc.contributor.author | Kostygov, Alexei Y. | |
dc.contributor.author | Konovalovas, Aleksandras | |
dc.contributor.author | Lye, Lon-Fye | |
dc.contributor.author | Dobson, Deborah E. | |
dc.contributor.author | Zangger, Haroun | |
dc.contributor.author | Fasel, Nicolas | |
dc.contributor.author | Butenko, Anzhelika | |
dc.contributor.author | Frolov, Alexander O. | |
dc.contributor.author | Votýpka, Jan | |
dc.contributor.author | d’Avila-Levy, Claudia M. | |
dc.contributor.author | Kulich, Pavel | |
dc.contributor.author | Moravcová, Jana | |
dc.contributor.author | Plevka, Pavel | |
dc.contributor.author | Rogozin, Igor B. | |
dc.contributor.author | Serva, Saulius | |
dc.contributor.author | Lukeš, Julius | |
dc.contributor.author | Beverley, Stephen M. | |
dc.contributor.author | Yurchenko, Vyacheslav | |
dc.date.accessioned | 2023-09-18T16:41:11Z | |
dc.date.available | 2023-09-18T16:41:11Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/115983 | |
dc.description.abstract | Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite Leishmania (Crithidia and Leptomonas), as well as plant-infecting PhytomonasLeptomonas pyrrhocoris was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as Leptomonas seymouri bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed "Leishbunyavirus" (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed "Leishbunyaviridae" Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus Leishmaniavirus (LRV1/2), implying that it was acquired at about the same time the Leishmania became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-20 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Academic Search Premier | |
dc.relation.isreferencedby | AGRICOLA | |
dc.relation.isreferencedby | Animal Behavior Abstracts | |
dc.relation.isreferencedby | Embase | |
dc.relation.isreferencedby | MEDLINE | |
dc.relation.isreferencedby | PsycInfo | |
dc.relation.isreferencedby | Zentralblatt MATH (zbMATH) | |
dc.source.uri | http://www.pnas.org/content/115/3/E506.full | |
dc.subject | FM01 - Biokatalitinių procesų modeliavimas / Modelling of biocatalytic processes | |
dc.title | Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 0 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | University of Ostrava | |
dc.contributor.institution | Washington University School of Medicine | |
dc.contributor.institution | Zoological Institute of the Russian Academy of Sciences | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | University of Lausanne | |
dc.contributor.institution | Charles University | |
dc.contributor.institution | Instituto Oswaldo Cruz | |
dc.contributor.institution | Veterinary Research Institute | |
dc.contributor.institution | Central European Institute of Technology – Masaryk University | |
dc.contributor.institution | National Institutes of Health | |
dc.contributor.institution | Vilniaus universitetas Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | University of South Bohemia | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | Bunyavirales | |
dc.subject.en | Trypanosomatidae | |
dc.subject.en | coevolution | |
dc.subject.en | coinfection | |
dc.subject.en | persistent virus infection | |
dcterms.sourcetitle | Proceedings of the National Academy of Sciences of the United States of America | |
dc.description.issue | no 3 | |
dc.description.volume | Vol. 115 | |
dc.publisher.name | National Academy of Sciences | |
dc.identifier.doi | 10.1073/pnas.1717806115 | |
dc.identifier.elaba | 25192088 | |