dc.contributor.author | Novickij, Vitalij | |
dc.contributor.author | Grainys, Audrius | |
dc.contributor.author | Lastauskienė, Eglė | |
dc.contributor.author | Kananavičiūtė, Rūta | |
dc.contributor.author | Pamedytytė, Dovilė | |
dc.contributor.author | Kalėdienė, Lilija | |
dc.contributor.author | Novickij, Jurij | |
dc.contributor.author | Miklavčič, Damijan | |
dc.date.accessioned | 2023-09-18T16:43:06Z | |
dc.date.available | 2023-09-18T16:43:06Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | (BIS)VGT02-000032585 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/116297 | |
dc.description.abstract | Electroporation is a phenomenon occurring due to exposure of cells to Pulsed Electric Fields (PEF) which leads to increase of membrane permeability. Electroporation is used in medicine, biotechnology, and food processing. Recently, as an alternative to electroporation by PEF, Pulsed ElectroMagnetic Fields (PEMF) application causing similar biological effects was suggested. Since induced electric field in PEMF however is 2–3 magnitudes lower than in PEF electroporation, the membrane permeabilization mechanism remains hypothetical. We have designed pilot experiments where Saccharomyces cerevisiae and Candida lusitaniae cells were subjected to single 100–250 μs electrical pulse of 800 V with and without concomitant delivery of magnetic pulse (3, 6 and 9 T). As expected, after the PEF pulses only the number of Propidium Iodide (PI) fluorescent cells has increased, indicative of membrane permeabilization. We further show that single sub-millisecond magnetic field pulse did not cause detectable poration of yeast. Concomitant exposure of cells to pulsed electric (PEF) and magnetic field (PMF) however resulted in the increased number PI fluorescent cells and reduced viability. Our results show increased membrane permeability by PEF when combined with magnetic field pulse, which can explain electroporation at considerably lower electric field strengths induced by PEMF compared to classical electroporation. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-10 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.rights | Laisvai prieinamas internete | |
dc.source.uri | http://www.nature.com/articles/srep33537 | |
dc.source.uri | https://talpykla.elaba.lt/elaba-fedora/objects/elaba:18366636/datastreams/MAIN/content | |
dc.subject | MC02 - Elektros ir elektroniniai įrenginiai bei sistemos / Electrical and electronic devices and systems | |
dc.title | Pulsed Electromagnetic Field assisted in vitro electroporation: a pilot study | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | Article number: 33537
CC Creative Commons Attribution 4.0 International License | |
dcterms.references | 66 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | University of Ljubljana | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.researchfield | T 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dcterms.sourcetitle | Scientific reports | |
dc.description.volume | Vol. 6 | |
dc.publisher.name | Nature Publishing Group | |
dc.publisher.city | London | |
dc.identifier.doi | 000383335600002 | |
dc.identifier.doi | 10.1038/srep33537 | |
dc.identifier.elaba | 18366636 | |