Rodyti trumpą aprašą

dc.contributor.authorJarockytė, Greta
dc.contributor.authorDaugėlaitė, Eglė
dc.contributor.authorStašys, Marius
dc.contributor.authorStatkutė, Urtė
dc.contributor.authorPoderys, Vilius
dc.contributor.authorTseng, Ting-Chen
dc.contributor.authorHsu, Shan-Hui
dc.contributor.authorKarabanovas, Vitalijus
dc.contributor.authorRotomskis, Ričardas
dc.date.accessioned2023-09-18T16:46:43Z
dc.date.available2023-09-18T16:46:43Z
dc.date.issued2016
dc.identifier.issn1422-0067
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/116537
dc.description.abstractThe uptake and distribution of negatively charged superparamagnetic iron oxide (Fe3O4) nanoparticles (SPIONs) in mouse embryonic fibroblasts NIH3T3, and magnetic resonance imaging (MRI) signal influenced by SPIONs injected into experimental animals, were visualized and investigated. Cellular uptake and distribution of the SPIONs in NIH3T3 after staining with Prussian Blue were investigated by a bright-field microscope equipped with digital color camera. SPIONs were localized in vesicles, mostly placed near the nucleus. Toxicity of SPION nanoparticles tested with cell viability assay (XTT) was estimated. The viability of NIH3T3 cells remains approximately 95% within 3–24 h of incubation, and only a slight decrease of viability was observed after 48 h of incubation. MRI studies on Wistar rats using a clinical 1.5 T MRI scanner were showing that SPIONs give a negative contrast in the MRI. The dynamic MRI measurements of the SPION clearance from the injection site shows that SPIONs slowly disappear from injection sites and only a low concentration of nanoparticles was completely eliminated within three weeks. No functionalized SPIONs accumulate in cells by endocytic mechanism, none accumulate in the nucleus, and none are toxic at a desirable concentration. Therefore, they could be used as a dual imaging agent: as contrast agents for MRI and for traditional optical biopsy by using Prussian Blue staining.eng
dc.formatPDF
dc.format.extentp. 1-13
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyMEDLINE
dc.relation.isreferencedbyDOAJ
dc.rightsLaisvai prieinamas internetelt
dc.source.urihttp://www.mdpi.com/1422-0067/17/8/1193
dc.subjectFM02 - Energijos šaltinių medžiagos ir technologijos / Materials and technologies of energy sources
dc.titleAccumulation and Toxicity of Superparamagnetic Iron Oxide Nanoparticles in Cells and Experimental Animals
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references40
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionNacionalinis vėžio institutas
dc.contributor.institutionNational Taiwan University
dc.contributor.institutionVilniaus Gedimino technikos universitetas Nacionalinis vėžio institutas
dc.contributor.institutionNacionalinis vėžio institutas Vilniaus universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldN 011 - Biofizika / Biophysics
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enMagnetic nanoparticles
dc.subject.enSPIONs
dc.subject.enIron oxide
dc.subject.enCellular uptake
dc.subject.enMRI-optical dual imaging
dc.subject.enOptical biopsy of tissues cells
dc.subject.enMultifunctional cancer diagnostics
dcterms.sourcetitleInternational Journal of Molecular Sciences
dc.description.volume17
dc.publisher.nameMDPI AG
dc.publisher.cityBasel
dc.identifier.doi10.3390/ijms17081193
dc.identifier.elaba18941885


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