dc.contributor.author | Kulys, Juozas | |
dc.contributor.author | Bratkovskaja, Irina | |
dc.date.accessioned | 2023-09-18T17:02:16Z | |
dc.date.available | 2023-09-18T17:02:16Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0039-9140 | |
dc.identifier.other | (BIS)VGT02-000016422 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/119184 | |
dc.description.abstract | The spectrophotometric method of antioxidants determination using recombinant laccase Polyporus pinsitus (rPpL) and Myceliophthora thermophila (rMtL) was developed. The method includes simultaneous oxidation of the antioxidant and high reactive laccase substrate producing chromophoric radical cation. As laccase substrates ABTS and other high reactive phenoxazine derivatives: 2-phenoxazin-10-yl-ethanol (PET), 3-phenoxazin-10-yl-propane-1-sulfonic acid (PPSA) and 3-phenoxazin-10-yl-propionic acid (PPA) were used. The kinetic data were analysed using a scheme of simultaneous oxidation of the antioxidant and the substrate. In a range of (0.9–7.3) × 10−6 M of Trolox the measurings recovered 91 and 99% of the antioxidant if ABTS and both laccases were used. The recovery varied between 82 and 124% if phenoxazine derivatives were used. The antioxidant activity determined in rich with antioxidants food samples, i.e. date-palm, black raisin, golden raisin, skin of red grape, dice of red grape, fitted the literature data. | eng |
dc.format | PDF | |
dc.format.extent | p. 526-531 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Biological Abstracts | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | BIOSIS Previews | |
dc.relation.isreferencedby | CAB Abstracts | |
dc.relation.isreferencedby | INSPEC | |
dc.relation.isreferencedby | MEDLINE | |
dc.relation.isreferencedby | Compendex | |
dc.relation.isreferencedby | ScienceDirect | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.title | Antioxidants determination with laccase | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 24 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas Biochemijos institutas | |
dc.contributor.institution | Biochemijos institutas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.en | Antioxidant | |
dc.subject.en | Trolox | |
dc.subject.en | Laccase | |
dc.subject.en | ABTS | |
dc.subject.en | Phenoxazine | |
dc.subject.en | Radical cation | |
dcterms.sourcetitle | Talanta | |
dc.description.issue | iss. 2 | |
dc.description.volume | Vol. 72 | |
dc.publisher.name | Elsevier | |
dc.publisher.city | Amsterdam | |
dc.identifier.doi | LBT02-000028762 | |
dc.identifier.doi | 10.1016/j.talanta.2006.11.011 | |
dc.identifier.elaba | 3817126 | |