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dc.contributor.authorJarockytė, Greta
dc.contributor.authorDapkutė, Dominyka
dc.contributor.authorKarabanovas, Vitalijus
dc.contributor.authorDaugmaudis, Justinas Vygintas
dc.contributor.authorIvanauskas, Feliksas
dc.contributor.authorRotomskis, Ričardas
dc.date.accessioned2023-09-18T17:06:52Z
dc.date.available2023-09-18T17:06:52Z
dc.date.issued2018
dc.identifier.issn0304-4165
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/119787
dc.description.abstractMonolayer cell cultures have been considered the most suitable technique for in vivo cellular experiments. However, a lot of cellular functions and responses that are present in natural tissues are lost in two-dimensional cell cultures. In this context, nanoparticle accumulation data presented in literature are often not accurate enough to predict behavior of nanoparticles in vivo. Cellular spheroids show a higher degree of morphological and functional similarity to the tissues.Accumulation and distribution of carboxylated CdSe/ZnS quantum dots (QDs), chosen as model nanoparticles, was investigated in cellular spheroids composed of different phenotype mammalian cells. The findings were compared with the results obtained in in vivo experiments with human tumor xenografts in immunodeficient mice. The diffusive transport model was used for theoretical nanoparticles distribution estimation.QDs were accumulated only in cells, which were localized in the periphery of cellular spheroids. CdSe/ZnS QDs were shown to be stable and inert; they did not have any side-effects for cellular spheroids formation. Penetration of QDs in both cellular spheroids and in vivo tumor model was limited. The mathematical model confirmed the experimental results: nanoparticles penetrated only 25 μm into cellular spheroids after 24 h of incubation.Penetration of negatively charged nanoparticles is limited not only in tumor tissue, but also in cellular spheroids.eng
dc.formatPDF
dc.format.extentp. 914-923
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyMEDLINE
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.source.urihttps://doi.org/10.1016/j.bbagen.2017.12.014
dc.subjectFM02 - Energijos šaltinių medžiagos ir technologijos / Materials and technologies of energy sources
dc.title3D cellular spheroids as tools for understanding carboxylated quantum dot behavior in tumors
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references39
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionNacionalinis vėžio institutas Vilniaus universitetas
dc.contributor.institutionNacionalinis vėžio institutas Vilniaus Gedimino technikos universitetas
dc.contributor.institutionVilniaus universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldN 011 - Biofizika / Biophysics
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.researchfieldN 009 - Informatika / Computer science
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.en3D spheroid cell culture
dc.subject.enCellular spheroids
dc.subject.enHanging drop
dc.subject.enNanoparticles, Quantum dots, Accumulation, Distribution, Penetration, Confocal microscopy, Mathematical model, Human tumor xenografts
dcterms.sourcetitleBiochimica et biophysica acta : general subjects
dc.description.issueiss. 4
dc.description.volumeVol. 1862
dc.publisher.nameElsevier
dc.publisher.cityAmsterdam
dc.identifier.doi2-s2.0-85041669351
dc.identifier.doi000427312900014
dc.identifier.doi10.1016/j.bbagen.2017.12.014
dc.identifier.elaba26453552


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