dc.contributor.author | Navakauskienė, Rūta | |
dc.contributor.author | Treigytė, Gražina | |
dc.contributor.author | Borutinskaitė, Veronika Viktorija | |
dc.contributor.author | Matuzevičius, Dalius | |
dc.contributor.author | Navakauskas, Dalius | |
dc.contributor.author | Magnusson, Karl-Eric | |
dc.date.accessioned | 2023-09-18T17:11:44Z | |
dc.date.available | 2023-09-18T17:11:44Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1874-3919 | |
dc.identifier.other | (BIS)VUB02-000045225 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/120319 | |
dc.description.abstract | Dystrobrevin is a dystrophin-related component of the dystrophin-associated protein complex (DAPC). Using alpha-dystrobrevin as indicator, we aimed to elucidate the interaction network of the DAPC with other proteins during apoptosis of promyelocytic HL-60 cells. The precise role(s) of DBs are not known, but we and others have shown that they play a role in intracellular signal transduction and cellular organization. Apoptosis was induced with etoposide in the absence or presence of Z-VAD to block caspase activity, and we then followed the cellular distribution of α-DB and its association with other proteins, using confocal imaging and cell fractions analyses after immune-precipitation with anti-α-DB and mass spectrometry. Confocal imaging revealed distinct spatial relocalizations of α-DB between the cell membrane, cytosol and nucleus after induction of apoptosis. The expression levels of the identified proteins were evaluated with computer-assisted image analysis of the gels. We thus identified associations with structural and transport proteins (tropomyosin, myosin), membrane (ADAM21, syntrophin), ER-Golgi (TGN51, eIF38) and nuclear (Lamins, ribonucleoprotein C1/C2) proteins. These results suggest that apoptosis-induction in HL-60 cells involves not only classical markers of apoptosis but also a network α-DB-associated proteins at the cell membrane, the cytoplasm and nucleus, affecting key cellular transport processes and cellular structure. | eng |
dc.format | PDF | |
dc.format.extent | p. 3291-3303 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | MEDLINE | |
dc.relation.isreferencedby | Elsevier Biobase | |
dc.relation.isreferencedby | BIOSIS Previews | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.source.uri | https://doi.org/10.1016/j.jprot.2012.03.041 | |
dc.title | Alpha-dystrobrevin and its associated proteins in human promyelocytic leukemia cells induced to apoptosis | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | Mokslo kryptis: Elektros ir elektronikos inžinerija (01T); Chemijos inžinerija (05T). | |
dcterms.references | 41 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus universitetas Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Linköping university, Sweden | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.researchfield | N 009 - Informatika / Computer science | |
dc.subject.en | Alpha-DB | |
dc.subject.en | Leukemic cells | |
dc.subject.en | Apoptosis | |
dcterms.sourcetitle | Journal of proteomics | |
dc.description.issue | no 11 | |
dc.description.volume | vol. 75 | |
dc.publisher.name | Elsevier BV | |
dc.publisher.city | Amsterdam | |
dc.identifier.doi | VGT02-000025762 | |
dc.identifier.doi | 10.1016/j.jprot.2012.03.041 | |
dc.identifier.elaba | 4637929 | |