dc.contributor.author | Rotomskis, Ričardas | |
dc.contributor.author | Jurgelėnė, Živilė | |
dc.contributor.author | Stankevičius, Mantas | |
dc.contributor.author | Stankevičiūtė, Milda | |
dc.contributor.author | Kazlauskienė, Nijolė | |
dc.contributor.author | Jokšas, Kęstutis | |
dc.contributor.author | Montvydienė, Danguolė | |
dc.contributor.author | Kulvietis, Vytautas | |
dc.contributor.author | Karabanovas, Vitalijus | |
dc.date.accessioned | 2023-09-18T17:12:35Z | |
dc.date.available | 2023-09-18T17:12:35Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0048-9697 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/120694 | |
dc.description.abstract | Due to colloidal instability even with protective coatings, nanoparticles tend to aggregate in complex environments and possibly interact with biota. In this study, visualization of quantum dots (QDs) interaction with rainbow trout (Oncorhynchus mykiss) embryos was performed. Studies on zebrafish (Danio rerio) and pearl gourami (Trichogaster leerii) embryos have shown that QDs interact with embryos in a general manner and their affects are independent on the type of the embryo. It was demonstrated that carboxylated CdSe/ZnS QDs (4 nM) were aggregating in accumulation media and formed agglomerates on the surface of fish embryos under 1–12 days incubation in deep-well water. Detailed analysis of QDs distribution on fish embryos surface and investigation of the penetration of QDs through embryo's membrane showed that the chorion protects embryos from the penetration through the chorion and the accumulation of nanoparticles inside the embryos. Confocal microscopy and spectroscopy studies on rainbow trout embryos demonstrated that QDs cause chorion damage, due to QDs aggregation on the surface of chorion, even the formation of the agglomerates at the outer part of the embryos and/or with the mucus were detected. Aggregation of QDs and formation of agglomerates on the outer part of the embryo's membrane caused the intervention of the aggregates to the chorion and even partially destroyed the embryo's chorion. The incorporation of QDs in chorion was confirmed by two methods: in living embryos from a 3D reconstruction view, and in slices of embryos from a histology view. The damage of chorion integrity might have adverse effects on embryonic development. Moreover, for the first time the toxic effect of QDs was separated from the heavy metal toxicity, which is most commonly discussed in the literature to the toxicity of the QDs. | eng |
dc.format | PDF | |
dc.format.extent | p. 1280-1291 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Embase | |
dc.relation.isreferencedby | MEDLINE | |
dc.relation.isreferencedby | Science Citation Index | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.source.uri | https://doi.org/10.1016/j.scitotenv.2018.04.206 | |
dc.subject | FM01 - Biokatalitinių procesų modeliavimas / Modelling of biocatalytic processes | |
dc.title | Interaction of carboxylated CdSe/ZnS quantum dots with fish embryos: Towards understanding of nanoparticles toxicity | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 82 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Nacionalinis vėžio institutas Vilniaus universitetas | |
dc.contributor.institution | Gamtos tyrimų centras | |
dc.contributor.institution | Gamtos tyrimų centras Vilniaus universitetas | |
dc.contributor.institution | Nacionalinis vėžio institutas | |
dc.contributor.institution | Nacionalinis vėžio institutas Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
dc.subject.researchfield | N 012 - Ekologija ir aplinkotyra / Ecology and environmental studies | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | Nanoparticles | |
dc.subject.en | Quantum dots aggregation and agglomeration on fish embryos | |
dc.subject.en | Chorion | |
dc.subject.en | Confocal fluorescence microscopy | |
dc.subject.en | 3D reconstruction, nanotoxicity | |
dcterms.sourcetitle | Science of the total environment | |
dc.description.volume | Vol. 635 | |
dc.publisher.name | Elsevier | |
dc.publisher.city | Amsterdam | |
dc.identifier.doi | 2-s2.0-85046041762 | |
dc.identifier.doi | 000436494400121 | |
dc.identifier.doi | 10.1016/j.scitotenv.2018.04.206 | |
dc.identifier.elaba | 27875717 | |