dc.contributor.author | Sereikaitė, Jolanta | |
dc.contributor.author | Bumelis, Vladas Algirdas | |
dc.date.accessioned | 2023-09-18T17:18:49Z | |
dc.date.available | 2023-09-18T17:18:49Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1895-1066 | |
dc.identifier.other | (BIS)VGT02-000017923 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/121842 | |
dc.description.abstract | The interaction between bovine serum albumin and the mono azo reactive dye Orange ZT has been investigated using absorption difference spectroscopy. The influence of pH and ionic strength of the solution on the stability of the dye-protein complex has been determined. At 25 degrees C, the complex dissociation constants were equal to 24.0, 28.0, 7.0, 11.0, 17.6 and 46.0 mu M at pH 7.0, 6.5, 6.0, 5.5, 5.0 and 4.3, respectively. In the presence of 0.1, 0.2, 0.3 M KCl, at pH 6.0 and 25 degrees C, the complex dissociation constants were 8.8, 20.0, 18.0 mu M, respectively. The protein-dye complex dissociation constants show that Orange ZT could be used as an affinity ligand for protein purification. | eng |
dc.format | PDF | |
dc.format.extent | p. 509-512 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | SpringerLink | |
dc.source.uri | https://www.degruyter.com/downloadpdf/j/chem.2008.6.issue-4/s11532-008-0066-8/s11532-008-0066-8.pdf | |
dc.title | Interaction of serum albumin with vinyl sulfonate azo dye | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 19 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.contributor.department | Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.en | Orange ZT | |
dc.subject.en | Serum albumin | |
dc.subject.en | Absorption difference spectroscopy | |
dc.subject.en | Affinity ligand | |
dcterms.sourcetitle | Central European journal of chemistry | |
dc.description.issue | iss. 4 | |
dc.description.volume | Vol. 6 | |
dc.publisher.name | Versita | |
dc.publisher.city | Warsaw | |
dc.identifier.doi | 000260469000003 | |
dc.identifier.doi | 10.2478/s11532-008-0066-8 | |
dc.identifier.elaba | 3847502 | |