dc.contributor.author | Buivydienė, Arida | |
dc.contributor.author | Liakina, Valentina | |
dc.contributor.author | Kashuba, Elena | |
dc.contributor.author | Norkūnienė, Jolita | |
dc.contributor.author | Jokubauskienė, Skirmantė | |
dc.contributor.author | Gineikienė, Eglė | |
dc.contributor.author | Valantinas, Jonas | |
dc.date.accessioned | 2023-09-18T17:28:21Z | |
dc.date.available | 2023-09-18T17:28:21Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1010-660X | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/123555 | |
dc.description.abstract | Abstract: Background and objectives: The hepatitis C virus (HCV) is the major causative agent of hepatocellular carcinoma (HCC) in the western world. The efficacy of surveillance programs for early detection of HCC is not satisfactory: many tumors are diagnosed at the late, incurable stages. Therefore, there is a need in reliable prognostic markers for the proper follow-up of HCV-positive patients. The aim of the present study was to assess the prognostic value of the uridine–cytidine kinase-like protein 1 (UCKL-1), a putative oncoprotein, together with genetically determined polymorphisms in the interleukin 28B (IL28B) gene (rs12979860, rs8099917) in the development of HCC in HCV-positive cirrhotic patients. Materials and Methods: We included 32 HCV cirrhotic patients, 21 (65.6%) of whom had HCC. The expression of UCKL-1 was assessed in liver tissue sections, using immunohistochemistry. For IL28B rs12979860 and rs8099917 genotype analysis, the corresponding genomic regions were amplified by polymerase chain reaction (PCR) with appropriate primers. Results: We have found that UCKL-1 expression was significantly increased in HCC (p = 0.003). The presence of rs8099917 TT single-nucleotide polymorphism (SNP) elevated the chances of HCC manifestation more than sevenfold (OR = 7.3, p = 0.0273). The presence of rs12979860 CC SNP also heightened HCC chances more than sevenfold (OR = 7.5, p = 0.0765). Moreover, in the HCC group, a combination of IL28B rs12979860 non-TT and rs8099917 TT genotypes was observed more often, compared with the non-HCC group. Other combinations of IL28B rs12979860 and rs8099917 SNIPs were associated with a reduced risk of HCC development, approximately at the same extent. Conclusions: The presence of IL28B rs8099917 TT and rs12979860 CC SNPs, but not the intensity of UCKL-1 expression, is strongly associated with increased chances of HCC development in HCV-positive cirrhotic patients. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-10 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.rights | Laisvai prieinamas internete | |
dc.source.uri | https://doi.org/10.3390/medicina54050067 | |
dc.source.uri | https://talpykla.elaba.lt/elaba-fedora/objects/elaba:31708809/datastreams/MAIN/content | |
dc.title | Impact of the uridine–cytidine kinase like-1 protein and IL28B rs12979860 and rs8099917 SNPs on the development of hepatocellular carcinoma in cirrhotic chronic hepatitis C patients — a pilot study | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |
dcterms.references | 48 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Vilniaus universitetas Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Karolinska Institutet RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas Vilniaus kolegija | |
dc.contributor.institution | Vilniaus universitetas Valstybinis patologijos centras | |
dc.contributor.institution | Vilniaus universitetas Vilniaus universiteto ligoninės Santaros klinikos | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | M 001 - Medicina / Medicine | |
dc.subject.vgtuprioritizedfields | FM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | Keywords: hepatitis C | |
dc.subject.en | liver cirrhosis | |
dc.subject.en | hepatocellular carcinoma | |
dc.subject.en | uridine–cytidine kinase like-1 | |
dc.subject.en | interleukin 28B | |
dcterms.sourcetitle | Medicina | |
dc.description.issue | no 67 | |
dc.description.volume | vol. 54 | |
dc.publisher.name | MDPI | |
dc.publisher.city | Basel | |
dc.identifier.doi | 10.3390/medicina54050067 | |
dc.identifier.elaba | 31708809 | |