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dc.contributor.authorStrainienė, Eglė
dc.contributor.authorČirkovas, Andrejus
dc.contributor.authorRadzevičius, Kostas
dc.contributor.authorLarsen, Kim Lambertsen
dc.contributor.authorSereikaitė, Jolanta
dc.contributor.authorBumelis, Vladas Algirdas
dc.date.accessioned2023-09-18T17:29:27Z
dc.date.available2023-09-18T17:29:27Z
dc.date.issued2009
dc.identifier.issn0141-8130
dc.identifier.other(BIS)VGT02-000019226
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/123761
dc.description.abstractCyclodextrins with different ring size and ring substituents were tested for recombinant mink and porcine growth hormones aggregation suppression in the refolding process from Escherichia coli inclusion bodies. Methyl-beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin show a positive effect on the aggregation suppression of both proteins. The influence of different methyl-beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin concentrations on the renaturation yield of both growth hormones was investigated. Moreover, methyl-beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin suppress not only folding-related, but also temperature-related aggregates formation of both proteins. Circular dichroism experiments (monitoring of protein solution turbidity by registering high tension voltage) showed that the onset temperature of aggregation of both growth hormones increased with increasing 2-hydroxypropyl-beta-cyclodextrin concentration. In conclusion, Cyclodextrins have perspectives in biotechnology of veterinary growth hormones not only for protein production, but also for its storage. (C) 2009 Elsevier B.V. All rights reserved.eng
dc.formatPDF
dc.format.extentp. 428-434
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyElsevier Biobase
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.titleAnti-aggregatory effect of cyclodextrins in the refolding process of recombinant growth hormones from Escherichia coli inclusion bodies
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references33
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionAalborg University, Department of Biotechnology, Chemistry & Environment
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.contributor.departmentChemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.enGrowth hormones
dc.subject.enRefolding
dc.subject.en69 Cyclodextrins
dcterms.sourcetitleInternational journal of biological macromolecules
dc.description.issueiss. 5
dc.description.volumeVol. 44
dc.publisher.nameElsevier
dc.publisher.cityNetherlands
dc.identifier.doi10.1016/j.ijbiomac.2009.03.005
dc.identifier.elaba3871813


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