dc.contributor.author | Ruzgys, Paulius | |
dc.contributor.author | Novickij, Vitalij | |
dc.contributor.author | Novickij, Jurij | |
dc.contributor.author | Šatkauskas, Saulius | |
dc.date.accessioned | 2023-09-18T17:29:36Z | |
dc.date.available | 2023-09-18T17:29:36Z | |
dc.date.issued | 2018 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/123783 | |
dc.description.abstract | Current electrotransfection protocols are well-established for decades and, as a rule, employ long micro-millisecond range electric field pulses to facilitate DNA transfer while application of nanosecond range pulses is limited. The purpose of this paper is to show that the transfection using ultrashort pulses is possible by regulating the pulse repetition frequency. We have used 200 ns pulses (10–18 kV/ cm) in bursts of ten with varied repetition frequency (1 Hz–1 MHz). The Chinese Hamster Ovary (CHO) cells were used as a cell model. Experiments were performed using green fluorescent protein (GFP) and luciferase (LUC) coding plasmids. Transfection expression levels were evaluated using flow cytometry or luminometer. It was shown that with the increase of frequency from 100 kHz to 1 MHz, the transfection expression levels increased up to 17% with minimal decrease in cell viability. The LUC coding plasmid was transferred more efficiently using high frequency bursts compared to single pulses of equivalent energy. The first proof of concept for frequency-controlled nanosecond electrotransfection was shown, which can find application as a new non-viral gene delivery method. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-8 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | PubMed | |
dc.relation.isreferencedby | Biological Abstracts | |
dc.relation.isreferencedby | Chemical abstracts | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | BIOSIS Previews | |
dc.relation.isreferencedby | Zoological Record | |
dc.rights | Laisvai prieinamas internete | |
dc.source.uri | https://www.nature.com/articles/s41598-018-33912-y | |
dc.source.uri | https://www.nature.com/articles/s41598-018-33912-y.pdf | |
dc.source.uri | https://talpykla.elaba.lt/elaba-fedora/objects/elaba:31888764/datastreams/MAIN/content | |
dc.title | Nanosecond range electric pulse application as a non-viral gene delivery method: proof of concept | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the
material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | |
dcterms.references | 67 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vytauto Didžiojo universitetas | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | T 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.vgtuprioritizedfields | MC0404 - Bionika ir biomedicinos inžinerinės sistemos / Bionics and Biomedical Engineering Systems | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | electroporation | |
dc.subject.en | gene delivery | |
dc.subject.en | pulsed power | |
dc.subject.en | electric field | |
dcterms.sourcetitle | Scientific reports | |
dc.description.volume | vol. 8 | |
dc.publisher.name | Nature Publishing Group | |
dc.publisher.city | London | |
dc.identifier.doi | 2-s2.0-85055072764 | |
dc.identifier.doi | 000447707900017 | |
dc.identifier.doi | 10.1038/s41598-018-33912-y | |
dc.identifier.elaba | 31888764 | |