dc.contributor.author | Vepštaitė-Monstavičė, Iglė | |
dc.contributor.author | Lukša, Juliana | |
dc.contributor.author | Konovalovas, Aleksandras | |
dc.contributor.author | Juozapaitė, Dovilė | |
dc.contributor.author | Stanevičienė, Ramunė | |
dc.contributor.author | Strazdaitė Žielienė, Živilė | |
dc.contributor.author | Serva, Saulius | |
dc.contributor.author | Servienė, Elena | |
dc.date.accessioned | 2023-09-18T17:32:19Z | |
dc.date.available | 2023-09-18T17:32:19Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1999-4915 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/124154 | |
dc.description.abstract | The Saccharomycetaceae yeast family recently became recognized for expanding of the repertoire of different dsRNA-based viruses, highlighting the need for understanding of their cross-dependence. We isolated the Saccharomyces paradoxus AML-15-66 killer strain from spontaneous fermentation of serviceberries and identified helper and satellite viruses of the family Totiviridae, which are responsible for the killing phenotype. The corresponding full dsRNA genomes of viruses have been cloned and sequenced. Sequence analysis of SpV-LA-66 identified it to be most similar to S. paradoxus LA-28 type viruses, while SpV-M66 was mostly similar to the SpV-M21 virus. Sequence and functional analysis revealed significant differences between the K66 and the K28 toxins. The structural organization of the K66 protein resembled those of the K1/K2 type toxins. The AML-15-66 strain possesses the most expressed killing property towards the K28 toxin-producing strain. A genetic screen performed on S. cerevisiae YKO library strains revealed 125 gene products important for the functioning of the S. paradoxus K66 toxin, with 85% of the discovered modulators shared with S. cerevisiae K2 or K1 toxins. Investigation of the K66 protein binding to cells and different polysaccharides implies the β-1,6 glucans to be the primary receptors of S. paradoxus K66 toxin. For the first time, we demonstrated the coherent habitation of different types of helper and satellite viruses in a wild-type S. paradoxus strain. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-19 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Embase | |
dc.relation.isreferencedby | MEDLINE | |
dc.relation.isreferencedby | CAB Abstracts | |
dc.relation.isreferencedby | AGORA | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.source.uri | https://doi.org/10.3390/v10100564 | |
dc.title | Saccharomyces paradoxus K66 killer system evidences expanded assortment of helper and satellite viruses | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 72 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Gamtos tyrimų centras | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Vilniaus universitetas Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Gamtos tyrimų centras Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
dc.subject.vgtuprioritizedfields | FM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components | |
dc.subject.ltspecializations | L101 - Agroinovacijos ir maisto technologijos / Agro-innovation and food technologies | |
dc.subject.en | dsRNA virus | |
dc.subject.en | killer system | |
dc.subject.en | Saccharomyces paradoxus | |
dc.subject.en | Totiviridae | |
dcterms.sourcetitle | Viruses | |
dc.description.issue | iss. 10 | |
dc.description.volume | vol. 10 | |
dc.publisher.name | MDPI | |
dc.publisher.city | Basel | |
dc.identifier.doi | 2-s2.0-85055080497 | |
dc.identifier.doi | 85055080497 | |
dc.identifier.doi | 000448821200052 | |
dc.identifier.doi | 10.3390/v10100564 | |
dc.identifier.elaba | 32181452 | |