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The influence of different glycosylation patterns on factor VII biological activity

dc.contributor.authorSutkevičiūtė, Ieva
dc.contributor.authorMistinienė, Edita
dc.contributor.authorSereikaitė, Jolanta
dc.contributor.authorBumelis, Vladas Algirdas
dc.date.accessioned2023-09-18T17:32:22Z
dc.date.available2023-09-18T17:32:22Z
dc.date.issued2009
dc.identifier.issn0300-9084
dc.identifier.other(BIS)VGT02-000019505
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/124161
dc.description.abstractIn this study the bioactivity of three differently glycosylated blood coagulation factor VII (FVII) variants (human plasma FVII, recombinant human FVII produced in CHO and BHK cell cultures) were analyzed and compared. Surface plasmon resonance studies of FVII interaction with soluble and full length TF together with FVII autoactivation assays revealed that BHK-derived FVII has the highest bioactivity, while human plasma and CHO-derived FVII showed very similar bioactivity. The affinity of FVII variants to TF correlates with FVII autoactivation rates - the higher the affinity, the faster the autoactivation rate.eng
dc.formatPDF
dc.format.extentp. 1123-1130
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScienceDirect
dc.relation.isreferencedbyElsevier Biobase
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S0300908409001576
dc.titleThe influence of different glycosylation patterns on factor VII biological activity
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references57
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionUAB "SICOR Biotech", Vilnius
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.contributor.departmentChemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering
dc.subject.researchfieldN 004 - Biochemija / Biochemistry
dc.subject.enFactor VII
dc.subject.enTissue factor
dc.subject.enSurface plasmon resonance
dc.subject.enAutoactivation
dcterms.sourcetitleBiochimie
dc.description.issueiss. 9
dc.description.volumeVol. 91
dc.publisher.nameElsevier
dc.identifier.doi10.1016/j.biochi.2009.05.015
dc.identifier.elaba3877469


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