dc.contributor.author | Ramonas, Eimantas | |
dc.contributor.author | Ratautas, Dalius | |
dc.contributor.author | Dagys, Marius | |
dc.contributor.author | Meškys, Rolandas | |
dc.contributor.author | Kulys, Juozas | |
dc.date.accessioned | 2023-09-18T17:47:52Z | |
dc.date.available | 2023-09-18T17:47:52Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0039-9140 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/126173 | |
dc.description.abstract | In this paper we report the development of a highly sensitive amperometric glycerol biosensor based on alcohol dehydrogenase from Pseudomonas putida immobilized on graphite electrode modified with carbon nanotubes and a redox mediator tetrathiafulvalene. The designed biosensor demonstrates very high sensitivity towards glycerol (29.2 ± 0.9 µA mM–1 cm–2), low limit of detection (18 µM), linear range from 0.05 to 1.0 mM, high selectivity and satisfactory stability. Biosensor has been successfully used for the determination of glycerol concentration in buffer solutions as well as in the human urine samples. Received results shows a satisfactory agreement with the control measurements carried out using colorimetric commercially available glycerol determination assay kit, thus developed biosensor can be successfully applied for measurements of glycerol concentration in human urine and may be a fast, attractive and non-invasive tool for the determination of glycerol. | eng |
dc.format | PDF | |
dc.format.extent | p. 333-339 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | INSPEC | |
dc.relation.isreferencedby | Elsevier Biobase | |
dc.relation.isreferencedby | BIOSIS Previews | |
dc.relation.isreferencedby | SciSearch | |
dc.relation.isreferencedby | Chemical abstracts | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.source.uri | https://doi.org/10.1016/j.talanta.2019.03.063 | |
dc.title | Highly sensitive amperometric biosensor based on alcohol dehydrogenase for determination of glycerol in human urine | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 44 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas Vilniaus universitetas | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
dc.subject.vgtuprioritizedfields | FM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | amperometric biosensor | |
dc.subject.en | alcohol dehydrogenase | |
dc.subject.en | carbon nanotubes | |
dc.subject.en | glycerol | |
dc.subject.en | tetrathiafulvalene | |
dcterms.sourcetitle | Talanta | |
dc.description.volume | vol. 200 | |
dc.publisher.name | Elsevier | |
dc.publisher.city | Amsterdam | |
dc.identifier.doi | 2-s2.0-85063165036 | |
dc.identifier.doi | 000468715300041 | |
dc.identifier.doi | 10.1016/j.talanta.2019.03.063 | |
dc.identifier.elaba | 35317752 | |