dc.contributor.author | Kulys, Juozas | |
dc.contributor.author | Tetianec, Lidija | |
dc.contributor.author | Bratkovskaja, Irina | |
dc.date.accessioned | 2023-09-18T17:48:15Z | |
dc.date.available | 2023-09-18T17:48:15Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 1860-6768 | |
dc.identifier.other | (BIS)VGT02-000021329 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/126293 | |
dc.description.abstract | Pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (PQQ-GDH) offers a variety of opportunities for applications, e.g. in highly sensitive biosensors and electrosynthetic reactions Here we report on the acceleration (up to 4 9 x 10(4)-fold) of enzymatic ferricyanide reduction by artificial redox mediators (enhancers). The reaction mechanism includes reduction of the PQQ-GDH by glucose followed by oxidation of the reduced PQQ cofactor with either ferricyanide or a redox mediator A synergistic effect occurs through the oxidation of a reduced mediator by ferricyanide Using kinetic description of the coupled reaction, the second order rate constant for the reaction of an oxidized mediator with the reduced enzyme cofactor (k(ox)) can be calculated For different mediators this value is 2 2 x 10(6)-1 6 x 10(8) M(-1)s(-1) at pH 7 2 and 25 degrees C However, no correlation of the rate constant with the midpoint redox potential of the mediator could be established For low-potential mediators the synergistic effect is proportional to the ratio of k(ox(med))/k(ox(ferricyanide)), whereas for the high-potential mediators the effect depends on both this ratio and the concentration of the oxidized mediator, which can be calculated from the Nernst equation The described effect can be applied in various ways, e g. for substrate reactivity determination, electrosynthetic PQQ cofactor regeneration or building of new highly sensitive biosensors. | eng |
dc.format | PDF | |
dc.format.extent | p. 822-828 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Embase | |
dc.relation.isreferencedby | Biological Abstracts | |
dc.relation.isreferencedby | VINITI | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | MEDLINE | |
dc.relation.isreferencedby | CAB Abstracts | |
dc.relation.isreferencedby | Chemical abstracts | |
dc.relation.isreferencedby | Biotechnology & Bioengineering Abstracts | |
dc.relation.isreferencedby | BIOSIS Previews | |
dc.relation.isreferencedby | Elsevier Biobase | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.source.uri | http://onlinelibrary.wiley.com/doi/10.1002/biot.201000119/epdf | |
dc.title | Pyrroloquinoline quinone-dependent carbohydrate dehydrogenase: Activity enhancement and the role of artificial electron acceptors | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | IDS Number: 645TG | |
dcterms.references | 28 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas Biochemijos institutas | |
dc.contributor.institution | Biochemijos institutas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.en | Ferricyanide | |
dc.subject.en | Glucose dehydrogenase | |
dc.subject.en | PQQ | |
dc.subject.en | Mediator | |
dcterms.sourcetitle | Biotechnology Journal | |
dc.description.issue | Iss.8 | |
dc.description.volume | Vol. 5 | |
dc.publisher.name | Wiley | |
dc.publisher.city | Germany | |
dc.identifier.doi | LBT02-000041291 | |
dc.identifier.doi | 000281488500005 | |
dc.identifier.doi | 10.1002/biot.201000119 | |
dc.identifier.elaba | 3912539 | |