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Alcohol oxidase from the yeast Pichia pastoris – a potential catalyst for organic synthesis

dc.contributor.authorDienys, Gervydas
dc.contributor.authorJarmalavičius, Saulius
dc.contributor.authorBudrienė, Saulutė
dc.contributor.authorČitavičius, Donaldas Jonas
dc.contributor.authorSereikaitė, Jolanta
dc.date.accessioned2023-09-18T18:53:13Z
dc.date.available2023-09-18T18:53:13Z
dc.date.issued2003
dc.identifier.issn1381-1177
dc.identifier.other(BIS)VGT02-000005112
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/132810
dc.description.abstractAlcohol oxidase (AO) from the methylotropic yeast Pichia pastoris was isolated and investigated. Wide substrate specificity is characteristic for this enzyme. Unbranched primary alcohols are effectively oxidized by AO to aldehydes, including propargyl alcohol, 2-chloroethanol, 2-cyanoethanol, leading to important synthetic intermediates. AO was immobilized by covalent linking to macroporous cellulose activated by glutaraldehyde, yield of immobilization 80%. Presence of two izoenzymes of AO was suggested from the pH activity dependence.eng
dc.formatPDF
dc.format.extentp. 47-49
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyChemical abstracts
dc.relation.isreferencedbyCompendex
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S1381117702001352
dc.titleAlcohol oxidase from the yeast Pichia pastoris – a potential catalyst for organic synthesis
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references9
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionBiotechnologijos institutas
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.researchfieldN 003 - Chemija / Chemistry
dc.subject.researchfieldN 004 - Biochemija / Biochemistry
dc.subject.enAlcohol oxidase
dc.subject.enMethylotropic yeast
dc.subject.enPichia pastoris
dc.subject.enImmobilization
dc.subject.enAldehyde
dcterms.sourcetitleJournal of molecular catalysis B : Enzymatic
dc.description.issueiss. 1-2
dc.description.volumeVol. 21
dc.publisher.nameElsevier
dc.publisher.cityAmsterdam
dc.identifier.doiLBT02-000003696
dc.identifier.doiVUB02-000011814
dc.identifier.doi10.1016/S1381-1177(02)00135-2
dc.identifier.elaba3621284


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