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Divinyl sulfone as a crosslinking reagent for oligomeric proteins

dc.contributor.authorSereikaitė, Jolanta
dc.contributor.authorBassus, Donata
dc.contributor.authorBobnis, Renata
dc.contributor.authorDienys, Gervydas
dc.contributor.authorBumelienė, Žana
dc.contributor.authorBumelis, Vladas Algirdas
dc.date.accessioned2023-09-18T18:53:27Z
dc.date.available2023-09-18T18:53:27Z
dc.date.issued2003
dc.identifier.issn1068-1620
dc.identifier.other(BIS)VGT02-000005394
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/132926
dc.description.abstractThe kinetics of the nucleophilic addition reactions of divinyl sulfone to amino groups of glycine and model proteins was studied in aqueous solution at 30°C. The rate constants for glycine, bovine serum albu-min, and a1-casein were (4.84±0.58)x10­¹, (2.97± 0.31)x10­², and (2.38 ± 0.49)x10­² M­¹s­¹, respectively. Divinyl sulfone was proposed as a crosslinking reagent for the qualitative detection of protein association in solution. The crosslinking capacity of divinyl sulfone was compared to that of 1,3,5-triacryloylhexahydro-s-tri- azine.eng
dc.format.extentp. 227-230
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbySpringerLink
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.source.urihttp://link.springer.com/article/10.1023%2FA%3A1023928314772#
dc.titleDivinyl sulfone as a crosslinking reagent for oligomeric proteins
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references23
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionVilniaus universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.contributor.departmentChemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering
dc.subject.researchfieldN 003 - Chemija / Chemistry
dc.subject.enDivinyl sulfone
dc.subject.enOligomeric proteins
dc.subject.enProtein crosslinking
dc.subject.en1,3,5-triacryloylhexahydro-s-triazine
dcterms.sourcetitleRussian Journal of Bioorganic Chemistry : translated from ''Bioorganicheskaya Khimiya''
dc.description.issueiss. 3
dc.description.volumevol. 29
dc.publisher.nameKluwer Academic/Plenum Publishers
dc.publisher.cityLondon
dc.identifier.doiVUB02-000014172
dc.identifier.doi000183622300003
dc.identifier.elaba3625199


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