Rodyti trumpą aprašą

dc.contributor.authorSutkevičiūtė, Ieva
dc.contributor.authorSereikaitė, Jolanta
dc.contributor.authorBumelis, Vladas Algirdas
dc.date.accessioned2023-09-18T19:48:07Z
dc.date.available2023-09-18T19:48:07Z
dc.date.issued2007
dc.identifier.other(BIS)VGT02-000014809
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/143458
dc.description.abstractThe antraquinone dye Cibacron blue F3G-A is widely used as a ligand in affinity chromatography systems. Cibacron blue F3G-A is able to bind most types of proteins. In spite of the extensive application, the binding mechanisms between Cibacron blue F3G-A and proteins remain poorly understood. The formation of the complexes between Cibacron blue F3G-A and two therapeutic proteins, recombinant human interferon-a2b and recombinant human growth hormone, was found earlier by applying gel-permeation chromatography in combination with the absorption difference spectroscopy. Kd of the complexes Cibacron blue F3G-A – interferon-a2b and Cibacron blue F3G-A – growth hormone were found to be equal to 0.9-1.5 µM and 5.6 µM, respectively, at pH 6.9 and 25 oC. To determine probable dye binding sites in interferon-a2b and growth hormone molecules, time resolved limited proteolysis and MALDI-TOF MS analysis of obtained peptide mixtures was used. Trypsin was chosen for the digestion of proteins. The addition of the dye influenced mass spectra. The occurrence of new peaks indicating peptide-dye complexes was observed. Analysis of peptide maps revealed that peptides A17HR19 and L20HQLAFDTYQEFEEAYIPK38 of human growth hormone, and R14TLMLLAQMR23 and D33RHDFGFPQEEFGNQFQK50 of human interferon-a2b exhibit affinity to Cibacron blue F3G-A.eng
dc.format.extentp. 104
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.titleAnalysis of cibacron blue f3g-a interaction with therapeutic proteins by maldi-tof mass spectrometry
dc.typeKonferencijos pranešimo santrauka / Conference presentation abstract
dc.type.pubtypeT2 - Konferencijos pranešimo tezės / Conference presentation abstract
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.contributor.departmentChemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dcterms.sourcetitle4th Nordic separation science society (NoSSS) International Conference, 26-29 August, Kaunas : abstract book and final program / Vytautas Magnus University
dc.publisher.nameVytautas Magnus University
dc.publisher.cityKaunas
dc.identifier.elaba3783387


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Rodyti trumpą aprašą