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dc.contributor.authorGasiūnienė, Monika
dc.contributor.authorPetkus, Gintautas
dc.contributor.authorMatuzevičius, Dalius
dc.contributor.authorNavakauskas, Dalius
dc.contributor.authorNavakauskienė, Rūta
dc.date.accessioned2023-09-18T19:50:11Z
dc.date.available2023-09-18T19:50:11Z
dc.date.issued2019
dc.identifier.issn2005-3606
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/143952
dc.description.abstractBackground and Objectives: Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) may be a valuable source for cardiovascular tissue engineering and cell therapy. The aim of this study is to verify angiotensin II and transforming growth factor-beta 1 (TGF-β1) as potential cardiomyogenic differentiation inducers of AF-MSCs. Methods and Results: AF-MSCs were obtained from amniocentesis samples from second-trimester pregnant women, isolated and characterized by the expression of cell surface markers (CD44, CD90, CD105 positive; CD34 negative) and pluripotency genes (OCT4, SOX2, NANOG, REX1). Cardiomyogenic differentiation was induced using different concentrations of angiotensin II and TGF-β1. Successful initiation of differentiation was confirmed by alterations in cell morphology, upregulation of cardiac genes-markers NKX2-5, TBX5, GATA4, MYH6, TNNT2, DES and main cardiac ion channels genes (sodium, calcium, potassium) as determined by RT-qPCR. Western blot and immunofluorescence analysis revealed the increased expression of Connexin43, the main component of gap junctions, and Nkx2.5, the early cardiac transcription factor. Induced AF-MSCs switched their phenotype towards more energetic and started utilizing oxidative phosphorylation more than glycolysis for energy production as assessed using Agilent Seahorse XF analyzer. The immune analysis of chromatin-modifying enzymes DNMT1, HDAC1/2 and Polycomb repressive complex 1 and 2 (PRC1/2) proteins BMI1, EZH2 and SUZ12 as well as of modified histones H3 and H4 indicated global chromatin remodeling during the induced differentiation. Conclusions: Angiotensin II and TGF-β1 are efficient cardiomyogenic inducers of human AF-MSCs; they initiate alterations at the gene and protein expression, metabolic and epigenetic levels in stem cells leading towards cardiomyocyte-like phenotype formation.eng
dc.formatPDF
dc.format.extentp. 251-264
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyBIOSIS Previews
dc.relation.isreferencedbyBiological Abstracts
dc.relation.isreferencedbyPubMed
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.source.urihttp://www.ijstemcell.com/journal/view.html?doi=10.15283/ijsc18126
dc.source.urihttps://doi.org/10.15283/ijsc18126
dc.titleAngiotensin II and TGF- β 1 induce alterations in human amniotic fluid-derived mesenchymal stem cells leading to cardiomyogenic differentiation initiation
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references40
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.facultyElektronikos fakultetas / Faculty of Electronics
dc.subject.researchfieldT 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering
dc.subject.researchfieldM 001 - Medicina / Medicine
dc.subject.researchfieldT 007 - Informatikos inžinerija / Informatics engineering
dc.subject.vgtuprioritizedfieldsIK0303 - Dirbtinio intelekto ir sprendimų priėmimo sistemos / Artificial intelligence and decision support systems
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enmyocytes
dc.subject.encardiac
dc.subject.enamniotic fluid
dc.subject.encell differentiation
dc.subject.enchromatin
dc.subject.enhistones
dcterms.sourcetitleInternational journal of stem cells
dc.description.issueiss. 2
dc.description.volumevol. 12
dc.publisher.nameKorean Society for Stem Cell Research
dc.publisher.citySeoul
dc.identifier.doi000476654800007
dc.identifier.doi10.15283/ijsc18126
dc.identifier.elaba40373835


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