dc.contributor.author | Strainienė, Eglė | |
dc.contributor.author | Sereikaitė, Jolanta | |
dc.contributor.author | Bumelis, Vladas Algirdas | |
dc.date.accessioned | 2023-09-18T19:54:10Z | |
dc.date.available | 2023-09-18T19:54:10Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 1572-3887 | |
dc.identifier.other | (BIS)VGT02-000015368 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/144685 | |
dc.description.abstract | L-Arginine was used to suppress the aggregation of recombinant mink and porcine growth hormones in the refolding process from E. coli inclusion bodies by solubilization–dilution protocol at high protein concentration and pH 8.0. The influence of l-arginine concentration on the renaturation yield of both proteins was investigated. l-Arginine effectively suppressed the precipitation of growth hormones during dilution, but did not inhibit soluble oligomers formation. The results of mink and porcine growth hormones purification from 4 g of biomass are presented. | eng |
dc.format | PDF | |
dc.format.extent | p. 547-555 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | SpringerLink | |
dc.source.uri | https://link.springer.com/article/10.1007%2Fs10930-007-9096-x | |
dc.title | L -Arginine suppresses aggregation of recombinant growth hormones in refolding process from E. coli inclusion bodies | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 47 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.contributor.department | Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering | |
dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
dc.subject.en | L-Arginine | |
dc.subject.en | Recombinant growth hormones | |
dc.subject.en | Refolding | |
dc.subject.en | Aggregation | |
dcterms.sourcetitle | The protein journal | |
dc.description.issue | no. 8 | |
dc.description.volume | Vol. 26 | |
dc.publisher.name | Springer | |
dc.publisher.city | New York | |
dc.identifier.doi | 000250722600004 | |
dc.identifier.doi | 10.1007/s10930-007-9096-x | |
dc.identifier.elaba | 3793818 | |