dc.contributor.author | Novickij, Vitalij | |
dc.contributor.author | Rembialkowska, Nina | |
dc.contributor.author | Staigvila, Gediminas | |
dc.contributor.author | Kulbacka, Julita | |
dc.date.accessioned | 2023-09-18T20:19:49Z | |
dc.date.available | 2023-09-18T20:19:49Z | |
dc.date.issued | 2020 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/148906 | |
dc.description.abstract | In the present study, we report the effects of extracellular medium conductivity on cell response in the context of sub-microsecond range (100 ns–900 ns) electroporation, calcium electroporation and cell size. The effects of 25 ns and microsecond range (100 μs) pulses were also covered. As a model, three different cancer cell lines of various size (C32, MCF-7/DX and MC38/0) were used and the results indicated different size-dependent susceptibility patterns to the treatment. The applied pulsed electric field (PEF) protocols revealed a significant decrease of cell viability when calcium electroporation was used. The dependence of calcium ion transport and finally the anticancer effect on the external medium conductivity was determined. It was shown that small differences in conductivity do not alter viability significantly, however, mostly affect the permeabilization. At the same, MC38/0 cell line was the least susceptible to calcium electroporation, while the C32 line the most. In all cases calcium electroporation was mostly dependent on the sensitivity of cells to electroporation and could not be effectively improved by the increase of CaCl2 concentration from 2 mM to 5 mM. Lastly, sub-microsecond PEF stimulated aquaporin-4 and VDAC1/Porin immunoreactions in all treated cells lines, which indicated that cell water balance is affected, ions exchange is increased and release of mitochondrial products is occurrent. | eng |
dc.format | PDF | |
dc.format.extent | p. 1-12 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | SAO/NASA Astrophysics Data System | |
dc.relation.isreferencedby | Dimensions | |
dc.relation.isreferencedby | DOAJ | |
dc.relation.isreferencedby | PubMed Central | |
dc.relation.isreferencedby | PubMed | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.rights | Laisvai prieinamas internete | |
dc.source.uri | https://doi.org/10.1038/s41598-020-60789-7 | |
dc.source.uri | https://www.nature.com/articles/s41598-020-60789-7.pdf | |
dc.source.uri | https://talpykla.elaba.lt/elaba-fedora/objects/elaba:52435188/datastreams/MAIN/content | |
dc.title | Effects of extracellular medium conductivity on cell response in the context of sub-microsecond range calcium electroporation | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.accessRights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |
dcterms.license | Creative Commons – Attribution – 4.0 International | |
dcterms.references | 33 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Wroclaw Medical University | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | T 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.vgtuprioritizedfields | MC0404 - Bionika ir biomedicinos inžinerinės sistemos / Bionics and Biomedical Engineering Systems | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | electroporation | |
dc.subject.en | calcium | |
dc.subject.en | electric fields | |
dcterms.sourcetitle | Scientific reports | |
dc.description.volume | vol. 10 | |
dc.publisher.name | Nature Publishing Group | |
dc.publisher.city | London | |
dc.identifier.doi | 000563055600013 | |
dc.identifier.doi | 10.1038/s41598-020-60789-7 | |
dc.identifier.elaba | 52435188 | |