Rodyti trumpą aprašą

dc.contributor.authorJarmalavičiūtė, Akvilė
dc.contributor.authorTunaitis, Virginijus
dc.contributor.authorStrainienė, Eglė
dc.contributor.authorAldonytė, Rūta
dc.contributor.authorRamanavičius, Arūnas
dc.contributor.authorVenalis, Algirdas
dc.contributor.authorMagnusson, Karl-Eric
dc.contributor.authorPivoriūnas, Augustas
dc.date.accessioned2023-09-18T20:28:21Z
dc.date.available2023-09-18T20:28:21Z
dc.date.issued2013
dc.identifier.issn0895-8696
dc.identifier.other(BIS)VUB02-000049092
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/150008
dc.description.abstractStem cells isolated from human adult tissues represent a promising source for neural differentiation studies in vitro. We have isolated and characterized stem cells from human exfoliated deciduous teeth (SHEDs). These originate from the neural crest and therefore particularly suitable for induction of neural differentiation. We here established a novel three-stage protocol for neural differentiation of SHEDs cells. After adaptation to a serum-free and neurogenic environment, SHEDs were induced to differentiate. This resulted in the formation of stellate or bipolar round-shaped neuron-like cells with subpopulations expressing markers of sensory neurons (Brn3a, peripherin) and glia (myelin basic protein). Commercial PCR array analyses addressed the expression profiles of genes related to neurogenesis and cAMP/calcium signalling. We found distinct evidence for the upregulation of genes regulating the specification of sensory (MAF), sympathetic (midkine, pleitrophin) and dopaminergic (tyrosine hydroxylase, Nurr1) neurons and the differentiation and support of myelinating and non-myelinating Schwann cells (Krox24, Krox20, apolipoprotein E). Moreover, for genes controlling major developmental signalling pathways, there was upregulation of BMP (TGF beta-3, BMP2) and Notch (Notch 2, DLL1, HES1, HEY1, HEY2) in the differentiating SHEDs. SHEDs treated according to our new differentiation protocol gave rise to mixed neuronal/glial cell cultures, which opens new possibilities for in vitro studies of neuronal and glial specification and broadens the potential for the employment of such cells in experimental models and future treatment strategies.eng
dc.formatPDF
dc.format.extentp. 307-317
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyPubMed
dc.relation.isreferencedbyChemical abstracts
dc.relation.isreferencedbyBIOSIS Previews
dc.relation.isreferencedbyScopus
dc.source.urihttps://doi.org/10.1007/s12031-013-0046-0
dc.titleA new experimental model for neuronal and glial differentiation using stem cells derived from human exfoliated deciduous teeth
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references45
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus universitetas Valstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras
dc.contributor.institutionValstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionLinköping university, Sweden
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldN 010 - Biologija / Biology
dc.subject.researchfieldN 003 - Chemija / Chemistry
dc.subject.enNeural differentiation
dc.subject.enGlial differentiation
dc.subject.enNeural crest
dc.subject.enMesenchymal stem cells
dc.subject.enSHED
dcterms.sourcetitleJournal of molecular neuroscience
dc.description.issueno 2
dc.description.volumevol. 51
dc.publisher.nameHumana Press Inc
dc.publisher.cityTotowa
dc.identifier.doiVGT02-000027724
dc.identifier.doi000324637200008
dc.identifier.doi10.1007/s12031-013-0046-0
dc.identifier.elaba6154831


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