dc.contributor.author | Labeikytė, Danutė | |
dc.contributor.author | Sereikaitė, Jolanta | |
dc.date.accessioned | 2023-09-18T20:28:21Z | |
dc.date.available | 2023-09-18T20:28:21Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1464-1801 | |
dc.identifier.other | (BIS)VUB02-000050889 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/150011 | |
dc.description.abstract | The influence of osmotic shock in the presence of exogenous osmolytes on the formation and composition of insoluble protein fraction in Escherichia coli was investigated. Interferon-α5 (IFN-α5) expressed in E. coli BL21(DE3) was used as a model protein. Cells were cultivated at three different temperatures of 25, 30, and 37°C. Two different osmolytes were used. Glycine as an amino acid metabolized by E. coli, or betaine which is not metabolized by cells, was added to the growth medium in the presence of salt. In both cases (i.e. when metabolized or non-metabolized amino acid was used), IFN-α5 formed the aggregates, except at 25°C of cultivation. Moreover, the differences in the quantitative composition of insoluble protein fraction were revealed by the proteomic analysis. The amount of some identified proteins increased, decreased, or did not change in the samples cultivated under osmotic shock in the presence of betaine as compared with the sample cultivated without salt and betaine in growth medium. | eng |
dc.format | PDF | |
dc.format.extent | p. 219-226 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | CABI Abstracts Databases | |
dc.relation.isreferencedby | Biobase | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Chemical abstracts | |
dc.relation.isreferencedby | MEDLINE | |
dc.title | Influence of osmotic shock on Escherichia coli insoluble protein fraction in the presence of exogenous osmolytes | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 38 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.contributor.department | Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
dc.subject.en | Inclusion bodies | |
dc.subject.en | Osmotic shock | |
dc.subject.en | Osmolytes | |
dc.subject.en | Proteomic analysis | |
dcterms.sourcetitle | Journal of molecular microbiology and biotechnology | |
dc.description.issue | no 3 | |
dc.description.volume | vol. 23 | |
dc.publisher.name | S. Karger AG | |
dc.publisher.city | Basel | |
dc.identifier.doi | VGT02-000027032 | |
dc.identifier.doi | 000318214100004 | |
dc.identifier.doi | 10.1159/000350214 | |
dc.identifier.elaba | 6153143 | |