Response reactions to the complex influence of radionuclides and heavy metals

Abstract

The aim of the work - to evaluate peculiarities of complex influence, frequently taking place in natural environment, of 90Sr or 137Cs with heavy metals and components of mineral nutrition on the growth of spring wheat seedlings. 10 days-old green wheat seedlings (Triticum aestivum L., ‘Nandu’) were grown in sand and nutritional medium with additions of 90Sr, 137Cs and heavy metals (mixture: Co(NO3)2, Cd2(NO3), ZnCl2, CuCl2, CrO3, Pb(NO3)2 and Ni(NO3)2). Obtained data show that growth of wheat seedlings shoots in nutritional medium with 90Sr in complex with NO3" significantly activates RNA-polymerase II (RNP-II) in the model system of isolated nuclei. The nutritional medium with 90Sr and heavy metals (HM) at the low concentrations of radionuclide activated RNP-II, at high concentrations - inhibited. 90Sr at high concentration (14.6 Bq ml-1) increased the growth inhibiting influence of HM. 137Cs on the background of NO3" activated the growth (lenght and weight) of wheat seedlings shoots, but on the background of HM growth activating influence of 137Cs weakened or disappeared. The influence of 90Sr and 137Cs on growth, weight and activity of RNP- II in the model system of the isolated nuclei depended on the nutritional medium and changed from activation to inhibition of the processes. Inhibition was characteristic for complex influence of 90Sr or 137Cs with heavy metals.

Darbo tikslas - įvertinti 90Sr ar 137Cs kompleksinio poveikio su mineralinės mitybos elementais ir sunkiaisiais metalais ypatybes vasarinių kviečių augimui. 10 parų žali kviečių (Triticum aestivum L. ‘Nandu’) daigai buvo auginami smėlyje mitybinėje terpėje keičiant azoto kiekį, su radionuklidų 90Sr ar 137Cs ir sunkiųjų metalų priedais mišinyje: Co(NO3)2, Cd(NO3)2, ZnCl2, CuCl2, CrO3, Pb(NO3)2 ir Ni(NO3)2. Pagal gautuosius rezultatus kviečių daigų augimas ir RNR-polimerazės II aktyvumas izoliuotų branduolių sistemoje priklauso nuo mitybinės terpės sudėties ir gali kisti nuo aktyvacijos iki inhibicijos. Pastaroji yra būdinga 90Sr arba 137Cs kompleksiniam poveikiui su sunkiaisiais metalais.