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Amperometric immunosensor for diagnosis of BLV infection

dc.contributor.authorKurtinaitienė, Bogumila
dc.contributor.authorVasiliauskienė, Dovilė
dc.contributor.authorLaurinavičius, Valdas Stanislovas
dc.contributor.authorRamanavičienė, Almira
dc.contributor.authorRamanavičius, Arūnas
dc.date.accessioned2023-09-18T20:28:42Z
dc.date.available2023-09-18T20:28:42Z
dc.date.issued2008
dc.identifier.issn0956-5663
dc.identifier.other(BIS)LBT02-000034130
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/150158
dc.description.abstractA new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.eng
dc.formatPDF
dc.format.extentp. 1547-1554
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyBiological Abstracts
dc.relation.isreferencedbyAcademic Search Premier
dc.relation.isreferencedbyMEDLINE
dc.relation.isreferencedbyCompendex
dc.relation.isreferencedbyChemical abstracts
dc.relation.isreferencedbyScienceDirect
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.source.urihttps://doi.org/10.1016/j.bios.2008.01.014
dc.titleAmperometric immunosensor for diagnosis of BLV infection
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references37
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionBiochemijos institutas Vilniaus Gedimino technikos universitetas
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionBiochemijos institutas
dc.contributor.institutionVilniaus universiteto Imunologijos institutas Vilniaus universitetas
dc.contributor.institutionVilniaus universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.researchfieldN 003 - Chemija / Chemistry
dc.subject.enImmunosensor
dc.subject.enNanoBioTechnology
dc.subject.enHRP
dc.subject.enRedox mediator
dc.subject.enElectrochemical sensor
dc.subject.enTMB
dc.subject.enFCA
dc.subject.enBLV
dcterms.sourcetitleBiosensors & bioelectronics
dc.description.issueiss. 10
dc.description.volumevol. 23
dc.identifier.doiVGT02-000016592
dc.identifier.doiVUB02-000030612
dc.identifier.doi10.1016/j.bios.2008.01.014
dc.identifier.elaba5821675


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