Rodyti trumpą aprašą

dc.contributor.authorNovickij, Vitalij
dc.contributor.authorZinkevičienė, Auksė
dc.contributor.authorMalyško-Ptašinskė, Veronika
dc.contributor.authorNovickij, Jurij
dc.contributor.authorKulbacka, Julita
dc.contributor.authorRembialkowska, Nina
dc.contributor.authorGirkontaitė, Irutė
dc.date.accessioned2023-09-18T20:34:16Z
dc.date.available2023-09-18T20:34:16Z
dc.date.issued2020
dc.identifier.issn1011-1344
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/150935
dc.description.abstractThe cell membrane permeabilization in electroporation studies is usually quantified using fluorescent markers such as propidium iodide (PI) or YO-PRO, while Chinese Hamster Ovary cell line frequently serves as a model. In this work, as an alternative, we propose a sensitive methodology for detection and analysis of electroporation phenomenon based on bioluminescence. Luminescent mice myeloma SP2/0 cells (transfected using Luciferase- pcDNA3 plasmid) were used as a cell model. Electroporation has been studied using the 0.1–5 μs × 250 and 100 μs × 1–8 pulsing protocols in 1–2.5 kV/cm PEF range. It was shown that the bioluminescence response is dependent on the cell permeabilization state and can be effectively used to detect even weak permeabilization. During saturated permeabilization the methodology accurately predicts the losses of cell viability due to irre- versible electroporation. The results have been superpositioned with permeabilization and pore resealing (1 h post-treatment) data using PI. Also, the viability of the cells was evaluated. Lastly, the SP2/0 tumors have been developed in BALB/C mice and the methodology has been tested in vivo using electrochemotherapy with bleomycin.eng
dc.formatPDF
dc.format.extentp. 1-9
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyScienceDirect
dc.relation.isreferencedbyChemical abstracts
dc.relation.isreferencedbyMetals Abstracts
dc.relation.isreferencedbyElsevier Biobase
dc.relation.isreferencedbyEmbase
dc.relation.isreferencedbyPubMed
dc.source.urihttps://www.sciencedirect.com/science/article/pii/S1011134420305169?via%3Dihub
dc.source.urihttps://reader.elsevier.com/reader/sd/pii/S1011134420305169?token=D501C8190B200245546055A99F1D09B273BCFFFB14C4FE3E39CDE30473A21904A2606C5C01481EF7BE59CDF55059B79E
dc.titleBioluminescence as a sensitive electroporation indicator in sub-microsecond and microsecond range of electrical pulses
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references50
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.institutionValstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras
dc.contributor.institutionWroclaw Medical University
dc.contributor.facultyElektronikos fakultetas / Faculty of Electronics
dc.subject.researchfieldT 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering
dc.subject.researchfieldN 010 - Biologija / Biology
dc.subject.researchfieldN 011 - Biofizika / Biophysics
dc.subject.studydirectionF05 - Biotechnologijos / Biotechnology
dc.subject.vgtuprioritizedfieldsMC0404 - Bionika ir biomedicinos inžinerinės sistemos / Bionics and Biomedical Engineering Systems
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enbioluminescence
dc.subject.enbleomycin
dc.subject.enelectrochemotherapy
dc.subject.enpermeabilization
dc.subject.entumors
dcterms.sourcetitleJournal of Photochemistry & Photobiology, B: Biology
dc.description.volumevol. 213
dc.publisher.nameElsevier
dc.publisher.cityLausanne
dc.identifier.doi000601341900003
dc.identifier.doi10.1016/j.jphotobiol.2020.112066
dc.identifier.elaba73688603


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