| dc.contributor.author | Rastenienė, Augustė | |
| dc.contributor.author | Gruškienė, Rūta | |
| dc.contributor.author | Sereikaitė, Jolanta | |
| dc.date.accessioned | 2023-09-18T20:37:22Z | |
| dc.date.available | 2023-09-18T20:37:22Z | |
| dc.date.issued | 2021 | |
| dc.identifier.issn | 2585-7290 | |
| dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/151414 | |
| dc.description.abstract | Ectoine and hydroxyectoine are well known extremolytes that accumulate in halophilic and halotolerant microorganisms. They are investigated as protein stabilizers and as excipients in protein formulations and biopharmaceuticals. However, their efect is quite controversial. In this work, the infuence of both extremolytes on mink growth hormone as a model protein was studied by fuorescence spectroscopy. The study revealed that the fuorescence quenching of protein tryptophan by ectoine and hydroxyectoine difers and is dependent on the pH value of solution. Moreover, the quenching mode by ectoine and hydroxyectoine was compared with a well-known quencher acrylamide. The diferences in the extremolytes interaction with protein revealed by fuorescence spectroscopy could be related to their diferent efect as protein stabilizers under various conditions. ectoine; hydroxyectoine; mink growth hormone; acrylamide; fluorescence quenching; sphere action static mechanism | eng |
| dc.format | PDF | |
| dc.format.extent | p. 2703-2712 | |
| dc.format.medium | tekstas / txt | |
| dc.language.iso | eng | |
| dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
| dc.relation.isreferencedby | Scopus | |
| dc.source.uri | https://doi.org/10.1007/s11696-021-01527-9 | |
| dc.source.uri | https://link.springer.com/content/pdf/10.1007/s11696-021-01527-9.pdf | |
| dc.title | Interaction of ectoine and hydroxyectoine with protein: fuorescence study | |
| dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
| dcterms.references | 63 | |
| dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
| dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
| dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
| dc.contributor.department | Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering | |
| dc.subject.researchfield | T 005 - Chemijos inžinerija / Chemical engineering | |
| dc.subject.vgtuprioritizedfields | FM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components | |
| dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
| dc.subject.en | ectoine | |
| dc.subject.en | hydroxyectoine | |
| dc.subject.en | mink growth hormone | |
| dc.subject.en | acrylamide | |
| dc.subject.en | fluorescence quenching | |
| dc.subject.en | sphere action static mechanism | |
| dcterms.sourcetitle | Chemical papers | |
| dc.description.volume | vol. 75 | |
| dc.publisher.name | Springer | |
| dc.publisher.city | Cham | |
| dc.identifier.doi | 000613041600001 | |
| dc.identifier.doi | 10.1007/s11696-021-01527-9 | |
| dc.identifier.elaba | 82598434 | |
