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dc.contributor.authorVersockienė, Justina
dc.contributor.authorBičkovas, Nedas
dc.contributor.authorSakalauskas, Andrius
dc.contributor.authorNovickij, Vitalij
dc.contributor.authorSmirnovas, Vytautas
dc.contributor.authorLastauskienė, Eglė
dc.date.accessioned2023-09-18T20:43:06Z
dc.date.available2023-09-18T20:43:06Z
dc.date.issued2021
dc.identifier.issn2076-3417
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/151977
dc.description.abstractPrions are misfolded, self-replicating, and transmissible proteins capable of causing different conditions that affect the brain and nervous system in humans and animals. Yeasts are the perfect model to study prion formation, dissemination, and the structure of protein aggregates. Yeast prions are related to stress resistance, cell fitness, and viability. Applying a pulsed electric field (PEF) as a factor capable of disintegrating the amyloid aggregates arises from the fact that the amyloid aggregates form via noncovalent bonds and stabilize via electrostatic interactions. In this research, we applied 2–26 kV/cm PEF delivered in sequences of 5 pulses of 1 ms duration to the Saccharomyces cerevisiae cell without prions and containing strong and weak variants of the [PSI+] prion (prion form of Sup35 translation termination factor). We determined that prions significantly increase cell survivability and resistance to PEF treatment. The application of PEF to the purified Sup35NM fibrils showed that the electric field causes significant reductions in the length of fibrils and the full disintegration of fibrils to Sup35 oligomers can be achieved in higher fields.eng
dc.formatPDF
dc.format.extentp. [1-10]
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyINSPEC
dc.relation.isreferencedbyCurrent Contents
dc.rightsLaisvai prieinamas internete
dc.source.urihttps://talpykla.elaba.lt/elaba-fedora/objects/elaba:89050268/datastreams/MAIN/content
dc.titleEffects of pulsed electric fields on yeast with prions and the structure of amyloid fibrils
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.licenseCreative Commons – Attribution – 4.0 International
dcterms.references47
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.facultyElektronikos fakultetas / Faculty of Electronics
dc.subject.researchfieldN 010 - Biologija / Biology
dc.subject.researchfieldT 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering
dc.subject.studydirectionD03 - Mikrobiologija / Microbiology
dc.subject.vgtuprioritizedfieldsMC0404 - Bionika ir biomedicinos inžinerinės sistemos / Bionics and Biomedical Engineering Systems
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enpulsed electric field
dc.subject.enprion elimination
dc.subject.enprotein electroporation
dc.subject.enyeast prions
dc.subject.enirreversible electroporation
dcterms.sourcetitleApplied sciences
dc.description.issueiss. 6
dc.description.volumevol. 11
dc.publisher.nameMDPI
dc.publisher.cityBasel
dc.identifier.doi000645821000001
dc.identifier.doi10.3390/app11062684
dc.identifier.elaba89050268


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