dc.contributor.author | Zaleskis, Gintaras | |
dc.contributor.author | Garberytė, Sima | |
dc.contributor.author | Pavliukevičienė, Božena | |
dc.contributor.author | Krasko, Jan Aleksander | |
dc.contributor.author | Skapas, Martynas | |
dc.contributor.author | Talaikis, Martynas | |
dc.contributor.author | Darinskas, Adas | |
dc.contributor.author | Žibutytė, Lavija | |
dc.contributor.author | Pašukonienė, Vita | |
dc.date.accessioned | 2023-09-18T20:44:02Z | |
dc.date.available | 2023-09-18T20:44:02Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0250-7005 | |
dc.identifier.other | (PMID)33952461 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/152164 | |
dc.description.abstract | Background/Aim: Liposomal Doxorubicin (lipDOX) and free Doxorubicin (DOX) are reported to exhibit similar antitumor efficacy. However, cellular internalization mechanisms of lipDOX are still a subject of controversy. Materials and Methods: Intact and permeabilized cells were exposed for short time to lipDOX and free DOX and drug intracellular content was evaluated by flow cytometry. Then, the antiproliferative capacities of lipDOX and free DOX were compared by the leukocyte nadir test in mice in vivo. Results: The fluorescence increase was 11.2-fold higher in intact cells and 19.7-fold higher in permeabilized cells after exposure to free DOX as compared to lipDOX. Mice injected with DOX showed pronounced antiproliferative activity with a leukocyte count decrease to 2.8±0.65 k/μl (p<0.01) – an effect significantly stronger than that in the lipDOX group. Conclusion: Intact and permeabilized cells internalize free DOX manifold faster than lipDOX. The LipDOX formulation does not induce a remarkable leukocyte nadir effect in vivo. | eng |
dc.format | PDF | |
dc.format.extent | p. 2363-2370 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.relation.isreferencedby | Scopus | |
dc.source.uri | https://doi.org/10.21873/anticanres.15011 | |
dc.title | Comparative evaluation of cellular uptake of free and liposomal doxorubicin following short term exposure | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 37 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Nacionalinis vėžio institutas | |
dc.contributor.institution | Valstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras | |
dc.contributor.institution | Nacionalinis vėžio institutas Vilniaus Gedimino technikos universitetas | |
dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | M 001 - Medicina / Medicine | |
dc.subject.researchfield | N 004 - Biochemija / Biochemistry | |
dc.subject.researchfield | N 003 - Chemija / Chemistry | |
dc.subject.researchfield | N 002 - Fizika / Physics | |
dc.subject.vgtuprioritizedfields | FM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | liposomal doxorubicin | |
dc.subject.en | apoptotic cell | |
dc.subject.en | drug leakage | |
dc.subject.en | drug uptake | |
dc.subject.en | pegylated liposomes | |
dcterms.sourcetitle | Anticancer research | |
dc.description.issue | iss. 5 | |
dc.description.volume | vol. 41 | |
dc.publisher.name | International Institute of Anticancer Research | |
dc.publisher.city | Attiki, Greece | |
dc.identifier.doi | 33952461 | |
dc.identifier.doi | 000648665800003 | |
dc.identifier.doi | 10.21873/anticanres.15011 | |
dc.identifier.elaba | 94551826 | |