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dc.contributor.authorVaškevičius, Laurynas
dc.contributor.authorMalūnavičius, Vilius
dc.contributor.authorJankunec, Marija
dc.contributor.authorLastauskienė, Eglė
dc.contributor.authorTalaikis, Martynas
dc.contributor.authorMikoliūnaitė, Lina
dc.contributor.authorManeikis, Andrius
dc.contributor.authorGudiukaitė, Renata
dc.date.accessioned2023-09-18T20:47:04Z
dc.date.available2023-09-18T20:47:04Z
dc.date.issued2023
dc.identifier.issn0013-9351
dc.identifier.other(crossref_id)149372583
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/152577
dc.description.abstractMicrobially induced calcite precipitation (MICP) is an efficient and eco-friendly technique that has attracted significant interest for resolving various problems in the soil (erosion, improving structural integrity and water retention, etc.), remediation of heavy metals, production of self-healing concrete or restoration of different concrete structures. The success of most common MICP methods depends on microorganisms degrading urea which leads to the formation of CaCO3 crystals. While Sporosarcina pasteurii is a well-known microorganism for MICP, other soil abundant microorganisms, such as Staphylococcus bacteria have not been thoroughly studied for its efficiency in bioconsolidation though MICP is a very important proccess which can ensure soil quality and health. This study aimed to analyze MICP process at the surface level in Sporosarcina pasteurii and a newly screened Staphylococcus sp. H6 bacterium as well as show the possibility of this new microorganism to perform MICP. It was observed that Staphylococcus sp. H6 culture precipitated 157.35 ± 3.3 mM of Ca2+ ions from 200 mM, compared to 176 ± 4.8 mM precipitated by S. pasteurii. The bioconsolidation of sand particles was confirmed by Raman spectroscopy and XRD analysis, which indicated the formation of CaCO3 crystals for both Staphylococcus sp. H6 and S. pasteurii cells. The water-flow test suggested a significant reduction in water permeability in bioconsolidated sand samples for both Staphylococcus sp. H6 and S. pasteurii. Notably, this study provides the first evidence that CaCO3 precipitation occurs on the surface of Staphylococcus and S. pasteurii cells within the initial 15–30 min after exposure to the biocementation solution. Furthermore, Atomic force microscopy (AFM) indicated rapid changes in cell roughness, with bacterial cells becoming completely coated with CaCO3 crystals after 90 min incubation with a biocementation solution. To our knowledge, this is the first time where atomic force microscopy was used to visualize the dynamic of MICP on cell surface.eng
dc.formatPDF
dc.format.extentp. 1-17
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyScopus
dc.source.urihttps://doi.org/10.1016/j.envres.2023.116588
dc.source.urihttps://talpykla.elaba.lt/elaba-fedora/objects/elaba:171825323/datastreams/COVER/content
dc.titleInsights in MICP dynamics in urease-positive Staphylococcus sp. H6 and Sporosarcina pasteurii bacterium
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references132
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionVilniaus universitetas Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.facultyElektronikos fakultetas / Faculty of Electronics
dc.subject.researchfieldN 010 - Biologija / Biology
dc.subject.researchfieldN 003 - Chemija / Chemistry
dc.subject.enMicrobial ureases
dc.subject.enMICP
dc.subject.enAtomic force microscopy
dc.subject.enBioconsolidation
dc.subject.enStaphylococcus
dc.subject.enSporosarcina
dcterms.sourcetitleEnvironmental research
dc.description.volumevol. 234
dc.publisher.nameAcademic Press Inc Elsevier Science
dc.publisher.citySan Diego, CA
dc.identifier.doi149372583
dc.identifier.doi001045951500001
dc.identifier.doi10.1016/j.envres.2023.116588
dc.identifier.elaba171825323


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