Rodyti trumpą aprašą

dc.contributor.authorAlizadeh, Mehdi
dc.contributor.authorHabach, Fayez
dc.contributor.authorMačiulis, Mykolas
dc.contributor.authorKontenis, Lukas
dc.contributor.authorBagdonas, Saulius
dc.contributor.authorKrouglov, Serguei
dc.contributor.authorBaranauskas, Vytautas
dc.contributor.authorBulotienė, Danutė
dc.contributor.authorKarabanovas, Vitalijus
dc.contributor.authorRotomskis, Ričardas
dc.contributor.authorAkens, Margarete
dc.contributor.authorBarzda, Virginijus
dc.date.accessioned2023-09-18T20:51:42Z
dc.date.available2023-09-18T20:51:42Z
dc.date.issued2023
dc.identifier.issn0006-3495
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/153192
dc.description.abstractPolarimetric second harmonic generation (SHG) microscopy imaging is employed to investigate the ultrastructural organization of biological and biomimetic partially oriented fibrillar structures. The linear polarization-in polarization-out SHG microscopy measurements are conducted with rat tail tendon, rabbit cornea, pig cartilage, and biomimetic meso-tetra(4-sulfonatophenyl)porphine (TPPS4) cylindrical aggregates, which represent different two- and three-dimensional (2D and 3D) configurations of C6 symmetry fibril structures in the focal volume (voxel) of the microscope. The polarization-in polarization-out imaging of rat tail tendon reveals that SHG intensity is affected by parallel/antiparallel arrangements of the fibers, and achiral (R) and chiral (C) susceptibility component ratio values change by tilting the tendon fibers out of image plane. The R ratio changes for the 2D crossing fibers observed in cornea tissue. The 3D crossing of fibers also affects R ratio in cartilage tissue. The distinctly different dependence of R on crossing and tilting of fibers is demonstrated in collagen and TPPS4 aggregates, due to the achiral molecular susceptibility ratio having values below and above 3, respectively. The polarimetric microscopy results correspond well with the analytical expressions of amplitude and R and C ratios dependence on the crossing angle of the fibers. The experimentally measured SHG intensity and R and C ratio maps are consistent with the computational modeling of various fiber configurations presented in the preceding article. The demonstrated SHG intensity and R and C ratio dependencies on fibril configurations provide the basis for interpreting polarimetric SHG microscopy images in terms of 3D ultrastructural organization of fibers in each voxel of the samples.eng
dc.formatPDF
dc.format.extentp. 1-13
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.rightsNeprieinamas
dc.titlePolarimetric second harmonic generation microscopy of partially oriented fibers II: Imaging study
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.references63
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionUniversity of Toronto Vilniaus universitetas
dc.contributor.institutionUniversity of Toronto
dc.contributor.institutionVilniaus universitetas
dc.contributor.institutionVilniaus universitetas Light Conversion
dc.contributor.institutionNacionalinis vėžio institutas
dc.contributor.institutionVilniaus universitetas Nacionalinis vėžio institutas Vilniaus Gedimino technikos universitetas
dc.contributor.institutionVilniaus universitetas Nacionalinis vėžio institutas
dc.contributor.institutionUniversity of Toronto University Health Network
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.subject.researchfieldN 011 - Biofizika / Biophysics
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.researchfieldT 008 - Medžiagų inžinerija / Material engineering
dc.subject.vgtuprioritizedfieldsFM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enSHG
dc.subject.enself assembling
dc.subject.enTPPS4
dcterms.sourcetitleBiophysical journal
dc.description.issueiss. 00
dc.description.volumevol. 00
dc.publisher.nameCell press
dc.publisher.cityCambridge
dc.identifier.doi10.1016/j.bpj.2023.08.015
dc.identifier.elaba175628691


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Rodyti trumpą aprašą