Rodyti trumpą aprašą

dc.contributor.authorKranjc, Matej
dc.contributor.authorPolajzer, Tamara
dc.contributor.authorNovickij, Vitalij
dc.contributor.authorMiklavcic, Damijan
dc.date.accessioned2023-12-22T07:06:37Z
dc.date.available2023-12-22T07:06:37Z
dc.date.issued2023
dc.identifier.issn1661-6596
dc.identifier.other(WOS_ID)001083007300001
dc.identifier.urihttps://etalpykla.vilniustech.lt/xmlui/handle/123456789/153742
dc.description.abstractHigh-Intensity Pulsed Electromagnetic Fields (HI-PEMF) treatment is an emerging noninvasive and contactless alternative to conventional electroporation, since the electric field inside the tissue is induced remotely by an externally applied pulsed magnetic field. Recently, HI-PEMF has been successfully used in the transfer of plasmid DNA and siRNA in vivo, with no or minimal infiltration of immune cells. In addition to gene electrotransfer, treatment with HI-PEMF has also shown potential for electrochemotherapy, where activation of the immune response contributes to the treatment outcome. The immune response can be triggered by immunogenic cell death that is characterized by the release of damage-associated molecular patterns (DAMPs) from damaged or/and dying cells. In this study, the release of the best-known DAMP molecules, i.e., adenosine triphosphate (ATP), calreticulin and high mobility group box 1 protein (HMBG1), after HI-PEMF treatment was investigated in vitro on three different cell lines of different tissue origin and compared with conventional electroporation treatment parameters. We have shown that HI-PEMF by itself does not cause the release of HMGB1 or calreticulin, whereas the release of ATP was detected immediately after HI-PEMF treatment. Our results indicate that HI-PEMF treatment causes no to minimal release of DAMP molecules, which results in minimal/limited activation of the immune response.eng
dc.formatPDF
dc.format.extentp. 1-14
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyPubMed
dc.relation.isreferencedbyMEDLINE
dc.source.urihttps://doi.org/10.3390/ijms241914607
dc.titleDetermination of the impact of high-intensity pulsed electromagnetic fields on the release of damage-associated molecular pattern molecules
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.licenseCreative Commons – Attribution – 4.0 International
dcterms.references69
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionUniversity of Ljubljana
dc.contributor.institutionValstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras Vilniaus Gedimino technikos universitetas
dc.contributor.facultyElektronikos fakultetas / Faculty of Electronics
dc.subject.researchfieldT 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering
dc.subject.researchfieldN 010 - Biologija / Biology
dc.subject.vgtuprioritizedfieldsFM0202 - Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components
dc.subject.ltspecializationsL105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies
dc.subject.enelectromagnetic field
dc.subject.enHI-PEMF
dc.subject.enimmune response
dc.subject.enDAMP
dc.subject.enATP
dc.subject.encalreticulin
dc.subject.enHMGB1
dcterms.sourcetitleInternational journal of molecular sciences
dc.description.issueiss. 19
dc.description.volumevol. 24
dc.publisher.nameMDPI
dc.publisher.cityBasel
dc.identifier.doi001083007300001
dc.identifier.doi153432811
dc.identifier.doi2-s2.0-85174735091
dc.identifier.doi85174735091
dc.identifier.doi1
dc.identifier.doi10.3390/ijms241914607
dc.identifier.elaba180473687


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