Iron oxide magnetic nanoparticles functionalized by nisin
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Date
2023Author
Gruškienė, Rūta
Kavleiskaja, Tatjana
Stanevičienė, Ramunė
Servienė, Elena
Sereikaitė, Jolanta
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Over the last decades iron oxide magnetic nanoparticles, mostly magnetite (Fe3O4) and maghemite (γ–Fe2O3), have increasingly attracted attention as an efficient tool in various fields of application [1]. Here, we present the preparation and characterization of iron oxide magnetic nanoparticles functionalized by nisin. Nisin is naturally produced by Lactococcus lactis subsp. lactis as a small cationic peptide composed of 34 amino acid residues and is a well-known bacteriocin approved as a food additive for food preservation [2]. Iron oxide magnetic nanoparticles were prepared by the co-precipitation method and characterized by X-ray diffraction method. Magnetic nanoparticles were stabilized by citric, ascorbic, gallic, or glucuronic acid coating and were functionalized by nisin using a simple and low-cost adsorption method (Fig. 1). Nisin loading was confirmed by FT-IR spectra, thermogravimetric analysis, and dynamic light scattering methods. Nisin-loaded iron oxide magnetic nanoparticles were stable for at least six weeks as judged by zeta-potential and hydrodynamic diameter measurements. The functionality of nisin-loaded iron oxide magnetic nanoparticles was demonstrated on Grampositive bacteria. Functionalized nanoparticles could find their application in innovative and emerging technologies as antimicrobials.