Oxygen electroreduction catalysed by laccase wired to gold nanoparticles via trinuclear copper cluster

Abstract

Specific wiring of biocatalysts par excellence, viz. redox enzymes, to the electrode can be exploited in fabrication of high-performance bioelectronic devices. Here we report oxygen electroreduction catalysed by Didymocrea sp. J6 laccase wired to the gold nanoparticles via trinuclear copper cluster. Bypassing the intramolecular electron transfer, which under certain conditions is the rate limiting step of oxygen bioelectroreduction, has resulted in fabrication of a high current density biocathode based on high redox potential laccase, which is able to operate in electrolytes at a broad pH range in the presence of high fluoride concentrations.