Analysis of cibacron blue F3G-A interaction with therapeutic proteins by MALDI-TOF mass spectrometry

Abstract

The formation of the complexes between Cibacron blue F3G-A and two therapeutic proteins, recombinant human interferon-2b and recombinant human growth hormone, was investigated. The method of time-resolved limited proteolysis coupled with MALDI-TOF mass spectrometry was used. The analysis of peptide maps revealed that A17HR19 and L20HQLAFDTYQEFEEAYIPK38 of hGH, and R14TLMLLAQMR23 and D33RHDFGFPQEEFGNQFQK50 of hIFN-2b, exhibit affinity to Cibacron blue F3G-A. Copyright © 2008 John Wiley & Sons, Ltd.