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Analysis of cibacron blue F3G-A interaction with therapeutic proteins by MALDI-TOF mass spectrometry

dc.contributor.authorSutkevičiūtė, Ieva
dc.contributor.authorSereikaitė, Jolanta
dc.contributor.authorBumelis, Vladas Algirdas
dc.date.accessioned2023-09-18T17:14:10Z
dc.date.available2023-09-18T17:14:10Z
dc.date.issued2008
dc.identifier.issn0269-3879
dc.identifier.other(BIS)VGT02-000017405
dc.identifier.urihttps://etalpykla.vilniustech.lt/handle/123456789/121049
dc.description.abstractThe formation of the complexes between Cibacron blue F3G-A and two therapeutic proteins, recombinant human interferon-2b and recombinant human growth hormone, was investigated. The method of time-resolved limited proteolysis coupled with MALDI-TOF mass spectrometry was used. The analysis of peptide maps revealed that A17HR19 and L20HQLAFDTYQEFEEAYIPK38 of hGH, and R14TLMLLAQMR23 and D33RHDFGFPQEEFGNQFQK50 of hIFN-2b, exhibit affinity to Cibacron blue F3G-A. Copyright © 2008 John Wiley & Sons, Ltd.eng
dc.formatPDF
dc.format.extentp. 1001-1007
dc.format.mediumtekstas / txt
dc.language.isoeng
dc.relation.isreferencedbyWiley InterScience
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)
dc.titleAnalysis of cibacron blue F3G-A interaction with therapeutic proteins by MALDI-TOF mass spectrometry
dc.typeStraipsnis Web of Science DB / Article in Web of Science DB
dcterms.accessRightsIDS Number: 354CN
dcterms.references70
dc.type.pubtypeS1 - Straipsnis Web of Science DB / Web of Science DB article
dc.contributor.institutionVilniaus Gedimino technikos universitetas
dc.contributor.facultyFundamentinių mokslų fakultetas / Faculty of Fundamental Sciences
dc.contributor.departmentChemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering
dc.subject.researchfieldT 005 - Chemijos inžinerija / Chemical engineering
dc.subject.enCibacron blue F3G-A
dc.subject.enRecombinant human growth hormone
dc.subject.enRecombinant interferon-2b
dc.subject.enMALDI-TOF mass spectrometry
dcterms.sourcetitleBiomedical chromatography
dc.description.issueiss. 9
dc.description.volumeVol. 22
dc.publisher.cityEngland
dc.identifier.doi000259616600009
dc.identifier.doi10.1002/bmc.1019
dc.identifier.elaba3836597


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