Glucose biosensor based on chitosan-gold and prussian blue-gold nanoparticles

Abstract

Amperometric glucose biosensors were constructed by codeposition of glucose oxidase (GOx) with chitosangold (chitosan-AuNP) on gold-Prussian Blue (Au-PB) nanoparticles modified glassy carbon electrodes (GCE). The high stability of the biosensors was achieved using electrochemically controllable in situ Au-PB film preparation following the film covering with GOx layer encrusted with chitosan-AuNP. The biosensors showed 95 % response time up to 10 s, sensitivity up to 9.5 μA M-1 cm-2 and linear calibration graph up to 3 mM of glucose.