Экспрессия и очистка бактериальной глюкозодегидрогеназы и гибридного белка GDH-ab в дрожжах Sacharomyces Cerevisi AE, выделение гомогенных белковых препаратов
Data
2010Autorius
Pilevičienė, Simona
Lebionka, Algimantas
Servienė, Elena
Metaduomenys
Rodyti detalų aprašąSantrauka
Eukaryotic expression system, bearing bacterial gene of glucose dehydrogenase alone or fused with amyloid peptide coding sequence, were constructed by the using gene engineering methods. Investigation of heterologous gene expression in yeast S. cerevisiae allowed to optimize cultivation of producents in order to achieve maximal amount of active protein. The maximal GDH activity in the membraneous fraction of secretion systems possesing transformants was detected. The level of the expressed in S. cerevisiae and purified on CM column GDH and GDH-ab protein reached 15-20mg from 1 g wet weight of yeast culture.