dc.contributor.author | Lastauskienė, Eglė | |
dc.contributor.author | Novickij, Vitalij | |
dc.contributor.author | Zinkevičienė, Auksė | |
dc.contributor.author | Girkontaitė, Irutė | |
dc.contributor.author | Paškevičius, Algimantas | |
dc.contributor.author | Švedienė, Jurgita | |
dc.contributor.author | Markovskaja, Svetlana | |
dc.contributor.author | Novickij, Jurij | |
dc.date.accessioned | 2023-09-18T18:50:48Z | |
dc.date.available | 2023-09-18T18:50:48Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1473-5504 | |
dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/132593 | |
dc.description.abstract | Candida lusitaniae and C. guilliermondii are perfect model organisms for the study of Candida genera behaviour in various conditions. Both of them are rare pathogens capable to cause candidiasis in the patients with weakened immune system and can undergo morphology switches related to the increased antifungal drug resistance. Candida genera yeasts are able to inhabit diverse range of ecological niches including space ships and space stations. During the long-term expeditions, astronauts are affected by various factors that can change the state immune system. In such conditions, the commensal usually non-pathogenic microorganisms can spread through the body of the host and cause infections. Weakened immune system and limited use of drugs in spaceships promote the search of the alternative methods for the biocontrol of microorganisms. Several studies demonstrate that microorganisms are altering their gene expression, physiology, morphology, pathogenicity and evolving resistance to the antifungals under microgravity conditions. Our research indicated that switch to the pseudohyphae morphology leads up 30-fold increased resistance to amphotericin B in C. lusitaniae and C. guilliermondii. Cultivation of yeasts in rotary cell culture system (RCCS) is related to the altered cell growth and resistance to the antifungal treatment. Our results showed that growth in the RCCS led to the extreme increase in cell resistance to amphotericin B as compared with the standard growth conditions. In our research, we applied electroporation for the biocontrol of two Candida species. C. lusitaniae and C. guilliermondii cells grown in RCCS exhibited significantly increased survivability after pulsed electric field (PEF) treatment in comparison with cells grown under routine conditions. We have shown that PEF bursts of 2.5–25 kV cm−1 of 100 μs × 8 duration display a dose-dependent permeabilization of both studied Candida species. Our research indicated that budding cells and pseudohyphae morphology cells, with increased resistance to amphotericin B, can be effectively inactivated after applying PEF higher than 15 kV cm−1. | eng |
dc.format | PDF | |
dc.format.extent | p. 405-411 | |
dc.format.medium | tekstas / txt | |
dc.language.iso | eng | |
dc.relation.isreferencedby | PubMed | |
dc.relation.isreferencedby | GEOBASE | |
dc.relation.isreferencedby | Current Contents | |
dc.relation.isreferencedby | BIOSIS Previews | |
dc.relation.isreferencedby | Biological Abstracts | |
dc.relation.isreferencedby | Scopus | |
dc.relation.isreferencedby | Science Citation Index Expanded (Web of Science) | |
dc.source.uri | https://doi.org/10.1017/S1473550418000332 | |
dc.title | Application of pulsed electric fields for the elimination of highly drug-resistant Candida grown under modelled microgravity conditions | |
dc.type | Straipsnis Web of Science DB / Article in Web of Science DB | |
dcterms.references | 47 | |
dc.type.pubtype | S1 - Straipsnis Web of Science DB / Web of Science DB article | |
dc.contributor.institution | Vilniaus universitetas | |
dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
dc.contributor.institution | Valstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras | |
dc.contributor.institution | Gamtos tyrimų centras Vilniaus universiteto ligoninės Santaros klinikos | |
dc.contributor.institution | Gamtos tyrimų centras | |
dc.contributor.faculty | Elektronikos fakultetas / Faculty of Electronics | |
dc.subject.researchfield | N 010 - Biologija / Biology | |
dc.subject.researchfield | T 001 - Elektros ir elektronikos inžinerija / Electrical and electronic engineering | |
dc.subject.researchfield | N 011 - Biofizika / Biophysics | |
dc.subject.researchfield | N 013 - Botanika / Botany | |
dc.subject.vgtuprioritizedfields | MC0404 - Bionika ir biomedicinos inžinerinės sistemos / Bionics and Biomedical Engineering Systems | |
dc.subject.ltspecializations | L105 - Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies | |
dc.subject.en | Altered morphology | |
dc.subject.en | biocontrol | |
dc.subject.en | Candida | |
dc.subject.en | electropermeabilization | |
dc.subject.en | microgravity | |
dc.subject.en | pseudohyphae | |
dcterms.sourcetitle | International journal of astrobiology | |
dc.description.issue | no. 5 | |
dc.description.volume | vol. 18 | |
dc.publisher.name | Cambridge University Press | |
dc.publisher.city | New York | |
dc.identifier.doi | 000480306100003 | |
dc.identifier.doi | 10.1017/S1473550418000332 | |
dc.identifier.elaba | 36164299 | |