Analysis of the physical homogeneity and biological activity of recombinant human interferon-gamma

Abstract

We describe here analysis of recombinant human interferon-gamma (HuIFN-γ) homogeneity and biological activity under storage conditions at a temperature of -20°, 37°, 50°C in absence and presence of protease inhibitors. Appearance of aggregate forms has been found to decreased significantly the biological activity of HuIFN-γ. The highest aggregation level and the lowest biological activity were established in protein solutions treated with PMSF and in the cases of incubation at 50°C.

Tirtas žmogaus rekombinantinio interferono-gama (HuIFN-γ) homogeniškumas ir biologinis aktyvumas saugant preparatą -20°, 37° ir 50°C temperatūroje su proteazių inhibitoriais ir be jų. Nustatyta,kad susidarę agregatai mažina HuIFN-γ antivirusinį aktyvumą. Labiausiai aktyvumas prarandamas PMSF tirpaluose. PMSF ir 50°C temperatūra skatina rekombinantinio baltymo agregaciją ir mažina jo aktyvumą.