| dc.contributor.author | Bumelis, Vladas Algirdas | |
| dc.contributor.author | Bumelienė, Žana | |
| dc.contributor.author | Gedminienė, Genovaitė | |
| dc.contributor.author | Smirnovas, Vytautas | |
| dc.contributor.author | Sereikaitė, Jolanta | |
| dc.contributor.author | Medelytė, Ineta | |
| dc.date.accessioned | 2023-09-18T20:19:41Z | |
| dc.date.available | 2023-09-18T20:19:41Z | |
| dc.date.issued | 2003 | |
| dc.identifier.issn | 1392-0146 | |
| dc.identifier.other | (BIS)LBT02-000003929 | |
| dc.identifier.uri | https://etalpykla.vilniustech.lt/handle/123456789/148875 | |
| dc.description.abstract | We describe here analysis of recombinant human interferon-gamma (HuIFN-γ) homogeneity and biological activity under storage conditions at a temperature of -20°, 37°, 50°C in absence and presence of protease inhibitors. Appearance of aggregate forms has been found to decreased significantly the biological activity of HuIFN-γ. The highest aggregation level and the lowest biological activity were established in protein solutions treated with PMSF and in the cases of incubation at 50°C. | eng |
| dc.description.abstract | Tirtas žmogaus rekombinantinio interferono-gama (HuIFN-γ) homogeniškumas ir biologinis aktyvumas saugant preparatą -20°, 37° ir 50°C temperatūroje su proteazių inhibitoriais ir be jų. Nustatyta,kad susidarę agregatai mažina HuIFN-γ antivirusinį aktyvumą. Labiausiai aktyvumas prarandamas PMSF tirpaluose. PMSF ir 50°C temperatūra skatina rekombinantinio baltymo agregaciją ir mažina jo aktyvumą. | lit |
| dc.format.extent | p. 51-53 | |
| dc.format.medium | tekstas / txt | |
| dc.language.iso | eng | |
| dc.source.uri | http://elibrary.lt/resursai/LMA/Biologija/B-51-1.pdf | |
| dc.title | Analysis of the physical homogeneity and biological activity of recombinant human interferon-gamma | |
| dc.title.alternative | Žmogaus rekombinantinio interferono-gama homogeniškumo ir biologinio aktyvumo analizė | |
| dc.type | Straipsnis kitame recenzuotame leidinyje / Article in other peer-reviewed source | |
| dcterms.references | 7 | |
| dc.type.pubtype | S4 - Straipsnis kitame recenzuotame leidinyje / Article in other peer-reviewed publication | |
| dc.contributor.institution | Vilniaus Gedimino technikos universitetas | |
| dc.contributor.faculty | Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences | |
| dc.contributor.department | Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering | |
| dc.subject.researchfield | N 010 - Biologija / Biology | |
| dc.subject.en | Human recombinant interferon-gamma | |
| dc.subject.en | Biological activity | |
| dc.subject.en | Protease inhibitors | |
| dcterms.sourcetitle | Biologija | |
| dc.description.issue | Nr. 2 | |
| dc.identifier.doi | VGT02-000005237 | |
| dc.identifier.elaba | 5212422 | |
