Browsing Moksliniai ir apžvalginiai straipsniai / Research and Review Articles by Department "Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering"
Now showing items 1-20 of 71
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A quantitative model of thermal stabilization and destabilization of proteins by ligands
(Biophysical Journal, 2008)Equilibrium binding ligands usually increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. High-throughput screening for the discovery of drug-like compounds uses an ... -
An optimization procedure for preparing aqueous CAR/HP-CD aggregate dispersions
(Molecules, 2021)β-Carotene is a very important molecule for human health. It finds a large application in the food industry, especially for the development of functional foods and dietary supplements. However, β-carotene is an unstable ... -
Analysis of cibacron blue F3G-A interaction with therapeutic proteins by MALDI-TOF mass spectrometry
(Biomedical chromatography, 2008)The formation of the complexes between Cibacron blue F3G-A and two therapeutic proteins, recombinant human interferon-2b and recombinant human growth hormone, was investigated. The method of time-resolved limited proteolysis ... -
Analysis of the physical homogeneity and biological activity of recombinant human interferon-gamma
(Biologija, 2003)We describe here analysis of recombinant human interferon-gamma (HuIFN-γ) homogeneity and biological activity under storage conditions at a temperature of -20°, 37°, 50°C in absence and presence of protease inhibitors. ... -
Anti-aggregatory effect of cyclodextrins in the refolding process of recombinant growth hormones from Escherichia coli inclusion bodies
(International journal of biological macromolecules, 2009)Cyclodextrins with different ring size and ring substituents were tested for recombinant mink and porcine growth hormones aggregation suppression in the refolding process from Escherichia coli inclusion bodies. ... -
Application of inulin for the formulation and delivery of bioactive molecules and live cells /
(Carbohydrate polymers., 2024.)Inulin is a fructan biosynthesized mainly in plants of the Asteraceae family. It is also found in edible vegetables and fruits such as onion, garlic, leek, and banana. For the industrial production of inulin, chicory and ... -
Bovine serum albumin-dye binding
(Acta Chromatographica, 2005)The interaction of the dye reactive bright red 6C with bovine serum albumin has been investigated. Formation of a complex between reactive bright red 6C and bovine albumin was detected by use of absorption difference ... -
Carotenoids: Considerations for their use in functional foods, nutraceuticals, nutricosmetics, supplements, botanicals, and novel foods in the context of sustainability, circular economy, and climate change
(Annual review of food science and technology, 2021)Carotenoids are versatile isoprenoids that are important in food quality and health promotion. There is a need to establish recommended dietary intakes/nutritional reference values for carotenoids. Research on carotenoids ... -
Congo red interaction with α-proteins
(Acta biochimica Polonica, 2006)The ability of Congo red to form complexes with α-proteins, human growth hormone and human interferon-α2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation ... -
Correlation between fructan exohydrolase activity and the quality of Helianthus tuberosus L. Tubers
(Agronomy-Basel, 2018)Jerusalem artichoke tubers have diverse applications in the food industry as well as in biotechnology. Their suitability depends mostly on the inulin content. Seasonal fluctuations of fructan exohydrolase activity responsible ... -
Determination of fructan exohydrolase activity in the crude extracts of plants
(Electronic journal of biotechnology, 2014)Background: The roots of chicory and the tubers of Jerusalem artichoke are used for the production of inulin. However, a quality of tubers and roots, i.e. the content of inulin, monosaccarides and disaccharides, depends ... -
Determination of the dissociation constant and stoichiometry of a complex of the protein interferon a-2b with Cibacron Blue F3G-A
(Journal of Analytical Chemistry : translated from Zhurnal Analiticheskoi Khimii, 2003)The nonlinear least-squares technique was used for determining the complexation parameters of the recombinant human cytokine interferon a-2b with a pseudobiospecific ligand, Cibacron Blue F3G-A. The dis- sociation constant ... -
Different effects of l-arginine on the heat-induced unfolding and aggregation of proteins
(Biologicals, 2011)Circular dichroism spectroscopy was used to study the effect of l-arginine on the temperature related unfolding and aggregation of three growth hormones, i.e. human, porcine and mink growth hormones, and human interferon-α2b. ... -
Dimerization of human growth hormone in the presence of Congo red
(Biologija, 2004)Absorption difference spectroscopy has been used to monitor Congo red interaction with human growth hormone. It has been shown that Congo red forms a complex with this α-protein. Formation of human growth hormone dimers ... -
Divinyl sulfone as a crosslinking reagent for oligomeric proteins
(Russian Journal of Bioorganic Chemistry : translated from ''Bioorganicheskaya Khimiya'', 2003)The kinetics of the nucleophilic addition reactions of divinyl sulfone to amino groups of glycine and model proteins was studied in aqueous solution at 30°C. The rate constants for glycine, bovine serum albu-min, and ... -
Effects of pulsed electric fields and mild thermal treatment on antimicrobial efficacy of nisin-loaded pectin nanoparticles for food preservation
(LWT - Food Science and Technology, 2020)Pulsed electric field (PEF) treatment is a common and primarily non-thermal sterilization technique that is used in food processing, which can be improved when combined with food-compatible antimicrobial agents such as ... -
Electroporation-assisted inactivation of Escherichia Coli using nisin-loaded pectin nanoparticles
(Innovative food science & emerging technologies, 2016)Pulsed electric fields (PEF) trigger the electroporation phenomenon in cell membranes, which has found many biotechnological applications including food processing. Nisin is a prominent lantibiotic widely used as a food ... -
European database of carotenoid levels in foods. Factors affecting carotenoid content
(Foods: Special Issue Natural Carotenoids as Functional Food Ingredients, 2021)Many studies indicate that diets including carotenoid-rich foods have positive effects on human health. Some of these compounds are precursors of the essential nutrient vitamin A. The present work is aimed at implementing ... -
Examination of dye-protein interaction by gel-permeation chromatography
(Biomedical chromatography : Wiley, 2006)The interaction of Cibacron blue F3G-A with two therapeutic proteins, recombinant human growth hormone and recombinant human interferon-2b, has been examined by applying gel-permeation chromatography in combination with ... -
Fucoidan as a carrier of antimicrobial peptide: Preparation and characterization of nisin-loaded particles /
(LWT-Food science and technology., 2024.)Fucoidan is an anionic sulphated polysaccharide found in brown seaweed. Due to its various biological activities, biodegradability, and biocompatibility, the application of fucoidan is increasing in various fields. The ...