Browsing Straipsniai kituose recenzuojamuose leidiniuose / Articles in other peer-reviewed sources by Department "Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering"

Now showing items 1-7 of 7

    • Analysis of the physical homogeneity and biological activity of recombinant human interferon-gamma 

      Bumelis, Vladas Algirdas; Bumelienė, Žana; Gedminienė, Genovaitė; Smirnovas, Vytautas; Sereikaitė, Jolanta; Medelytė, Ineta (Biologija, 2003)
      We describe here analysis of recombinant human interferon-gamma (HuIFN-γ) homogeneity and biological activity under storage conditions at a temperature of -20°, 37°, 50°C in absence and presence of protease inhibitors. ...

    • Dimerization of human growth hormone in the presence of Congo red 

      Sereikaitė, Jolanta; Strainienė, Eglė; Bumelienė, Žana; Morkūnas, Mindaugas; Gedminienė, Genovaitė; Bumelis, Vladas Algirdas (Biologija, 2004)
      Absorption difference spectroscopy has been used to monitor Congo red interaction with human growth hormone. It has been shown that Congo red forms a complex with this α-protein. Formation of human growth hormone dimers ...

    • High level expression of recombinant bovine growth hormone 

      Morkūnas, Mindaugas; Gedminienė, Genovaitė; Sereikaitė, Jolanta; Bumelienė, Žana; Bumelis, Vladas Algirdas (Biologija, 2004)
      A high level biosynthesis of bovine growth hormone (bGH) was achieved in the E. coli BL21 (DE3) strain through the construction of pET21a/bGH plasmid. The plasmid carrying a sequence of bGH was used as a DNA template. The ...

    • Investigation of thermal stability of recombinant human interferon-gamma 

      Bumelis, Vladas Algirdas; Bumelienė, Žana; Gedminienė, Genovaitė; Smirnovas, Vytautas; Sereikaitė, Jolanta; Medelytė, Ineta (Biologija, 2002)
      Our studies in the structure and biological function of recombinant human interferon gamma (IFN-y) showed that IFN-y was stable at -20 C if to analyse the quantity of its monomeric form (MW 17240 Da). The protein aggregated ...

    • Separation of recombinant porcine growth hormone monomer from dimer and other oligomers in the production process from E. coli inclusion bodies 

      Sereikaitė, Jolanta; Bumelis, Vladas Algirdas (Biologija, 2006)
      Porcine growth hormone expressed in E. coli accumulates as inclusion bodies. After the refolding process, besides the monomer, also a dimer and higher oligomers of this protein were found. A successful application of ...

    • Studies on interferon-gamma - Cibacron blue F3GA interaction 

      Sereikaitė, Jolanta; Bumelienė, Žana; Bumelis, Vladas Algirdas; Smirnovas, Vytautas; Gedminienė, Genovaitė; Braziūnaitė, Lina; Bajorūnaitė, Laima (Biologija, 2003)
      Interaction between Cibacron blue F3GA and interferon - gamma in the presence of Tween 20 has been investigated by difference spectroscopy. Titration curves show the existence of different kinds of dye-binding sities. ...

    • Дивинилсульфон как сшивающий реагент для олигомерных белков 

      Sereikaitė, Jolanta; Bassus, Donata; Bobnis, Renata; Dienys, Gervydas; Bumelienė, Žana; Bumelis, Vladas Algirdas (Биоорганическая химия, 2003)
      Исследована кинетика реакции нуклеофильного присоединения дивинилсульфона к аминогруппам глицина и модельных белков в водном растворе при 30°C. Константы рекции для глицина, бычьего сывороточного альбумина и a1-казеина ...