Electroporation cuvette with integrated electrodes for high gradient electric field generation

Abstract

Electroporation is widely used in various biomedical and biotechnological applications, while homogeneous electric fields are usually required for successful treatment. In this work, a model of electroporation cuvette with integrated center electrode for generation of high gradient electric fields is analyzed using finite element method (FEM) simulations. Such a configuration potentially enables induction of dielectrophoretic force inside the effective volume of the cuvette before or after the high-power electroporation pulses. The results may be useful in further development of electrotransfer methods based on high frequency electric fields.